Zebrafish larvae submitted to simulated microgravity on a clinostat (CLINO) at 5dpf for one day
Description
The general objective of the study was to determine modulation of gene expression by environmental factors specifically simulation of microgravity with a special emphasis on bone formation. For this reason the specific period of treatment was chosen between 5-6 days post-fertilization (dpf) when bone formation and calcification are taking place. Zebrafish larvae were placed at 5 dpf into a clinostat (CLINO) for 24 hours which was shown to simulate microgravity by the specific rotational movements it generates. We show that CLINO exposure causes a clear decrease of bone formation as illustrated by cranial skeleton staining of the bone matrix by Alizarin Red by morphometric analysis of the resulting images. Thus a whole genome micro-array experiment was conducted to identify genes that may be involved in the observed effect on bone formation.
Resources
| Name |
Format |
Description |
Link |
|
21 |
GeneLab Study Page |
https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-76 |
Tags
- normalization-data-transformation
- p-mtab-48672
- p-mtab-48667
- p-mtab-48668
- p-mtab-48671
- treatment-protocol
- nucleic-acid-hybridization
- rna-extraction
- labeling
- growth-protocol
- p-mtab-48670
- p-mtab-48673
- array-scanning-and-feature-extraction
- p-mtab-48669
- stress