Using fecal DNA metabarcoding to test if diet of migratory birds is correlated with Pb exposure level at a contaminated site in north Idaho: rbcL dataset
Description
Fecal samples from migratory birds were collected from Pb contaminated wetlands in the Bunker Hill Lower Basin Coeur ’d Alene River Watershed and from a Hepton Lake reference area St. Joe River Watershed during 2022-2024. Chloroplast rbcL metabarcoding was used to identify plant taxa recently consumed by the birds.
rbcL primers with Illumina adaptors:
RBCL-F TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGYGATGGACTTACNAGTCTTGATCGTTACAAAGG
RBCL-R GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGNCCATAYTTRTTCAATTTATCTCTTTCAACTTGGATNCC
Also, mitochondrial CO1 metabarcoding was used to confirm bird taxa from which samples were collected, which can be found at DOI: 10.23719/1532394
Resources
| Name |
Format |
Description |
Link |
|
0 |
|
https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA1277348 |
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53 |
Bird_rbcL_SRA_metadata_Final.xlsx |
https://pasteur.epa.gov/uploads/10.23719/1532393/Bird_rbcL_SRA_metadata_Final.xlsx |
Tags
- birds
- rbcl
- amplicon-sequencing
- pb-toxicity