Using fecal DNA metabarcoding to test if diet of migratory birds is correlated with Pb exposure level at a contaminated site in north Idaho: rbcL dataset

Fecal samples from migratory birds were collected from Pb contaminated wetlands in the Bunker Hill Lower Basin Coeur ’d Alene River Watershed and from a Hepton Lake reference area St. Joe River Watershed during 2022-2024. Chloroplast rbcL metabarcoding was used to identify plant taxa recently consumed by the birds.

rbcL primers with Illumina adaptors:
RBCL-F TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGYGATGGACTTACNAGTCTTGATCGTTACAAAGG
RBCL-R GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGNCCATAYTTRTTCAATTTATCTCTTTCAACTTGGATNCC

Also, mitochondrial CO1 metabarcoding was used to confirm bird taxa from which samples were collected, which can be found at DOI: 10.23719/1532394