Using fecal DNA metabarcoding to test if diet of migratory birds is correlated with Pb exposure level at a contaminated site in north Idaho: CO1 dataset
Description
Fecal samples from migratory birds were collected from contaminated wetlands in the Bunker Hill Lower Basin Coeur ’d Alene River Watershed and from a Hepton Lake reference area St. Joe River Watershed during 2022-2024. Mitochondrial CO1 metabarcoding was used to confirm bird taxa from which samples were collected.
CO1 primers with Illumina adaptors:
CO1-F TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAACATTCAGCCATCTTACCCGTG
CO1-R GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGACAGGGAGTGATAGRAGKAGTAGG
Also, chloroplast rbcL metabarcoding was used to identify plant taxa recently consumed by the birds, which can be found at DOI: 10.23719/1532393
Resources
| Name |
Format |
Description |
Link |
|
0 |
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https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA1277348 |
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53 |
Bird_CO1_SRA_metadata_Final.xlsx |
https://pasteur.epa.gov/uploads/10.23719/1532394/Bird_CO1_SRA_metadata_Final.xlsx |
Tags
- birds
- co1
- amplicon-sequencing
- pb-toxicity