Unified Field Study 3 (UFS-3)

Description

The Unified Field Studies (UFS), established by the Algae Testbed Public-Private Partnership (ATP3), produced data on the effect of environmental and process conditions on algal growth rates and algal composition. The goal of the third Unified Field Study (UFS-3), performed June to September 2014, was to generate a robust inter-testbed dataset in algal biomass and lipid productivity and yield for two different algal strains (Nannochloropsis oceanica KA32 and Chlorella vulgaris LRB-AZ-1201) as a function of different regional, and environmental conditions among sites by controlling non-geographical-related variables including production systems, production process and protocols, system scale, and algae strain. In UFS-3 we compared the effect on productivity of three harvests per week with multiple dilution rates vs. one harvest per week with one dilution rate using both algal strains.

For more information about the UFS experiments see the reference resources 0.0 UFS-1 Experiment OpenEI Wiki Page, 0.1 ATP3 OpenEI Wiki Page, 0.2 Report on the Use of ATP3 UFS Data, and 0.3 ATP3 UFS Journal Article below.

Resources

Name Format Description Link
5.2 UFS-3 Compositional Analysis Data.csv CSV The data include the lipid (FAMELipids.AF), protein (Protein.AF) and carbohydrate (Carbohydrates.AF) content of biomass harvested from the ponds throughout the cultivation experiment and immediately freeze-dried. All data presented here are averaged over technical replicates and expressed as percent (%) on an ash-free dry weight basis of harvested biomass. Some data points are missing due to either data not meeting the rigorous QC requirements or experimental errors during data collection. Open resource
5.1 Compositional Data Definitions.pdf PDF Definition of terms and units for the Compositional Data provided in 5.2. Open resource
2.1 Harvest Data Definitions.pdf PDF Definition of terms and units for the Harvest Data provided in 2.2 and 2.3. Open resource
2.3 UFS-3 LRB-AZ-1201 Harvest Data.csv CSV These data include date, strain, batch and source ID, pond treatment, and depth (cm). Harvest data include harvest number, time between harvests, harvest volume (L), amount harvested (g), ash-free dry weight (AFDW; g L−1) at the time of harvest, and an indication of a pond crash. Open resource
2.2 UFS-3 KA32 Harvest Data.csv CSV These data include date, strain, batch and source ID, pond treatment, and depth (cm). Harvest data include harvest number, time between harvests, harvest volume (L), amount harvested (g), ash-free dry weight (AFDW; g L−1) at the time of harvest, and an indication of a pond crash. Open resource
3.4 Daily Averaged Instrumentation Data Definitions.pdf PDF Definition of terms and units for the Daily Averaged Instrumentation Data provided in 3.5 and 3.6. Open resource
3.6 UFS-3 LRB-AZ-1201 Daily Averaged Instrumentation Data.csv CSV These data include the pH, oxidation-reduction potention (ORP, mv), pond water temperature (C), pond conductivity (ms.cm), dissolved oxygen concentration (DO, mg/L), dissolved oxygen saturation (%), salinity (g/L), and photosynthetically active radiation (PAR, umol/m2/s). The PAR sensor was connected to only one of the ponds. Open resource
3.5 UFS-3 K32 Daily Averaged Instrumentation Data.csv CSV These data include the pH, oxidation-reduction potention (ORP, mv), pond water temperature (C), pond conductivity (ms.cm), dissolved oxygen concentration (DO, mg/L), dissolved oxygen saturation (%), salinity (g/L), and photosynthetically active radiation (PAR, umol/m2/s). The PAR sensor was connected to only one of the ponds. Open resource
3.1 Instrumentation Data Definitions.pdf PDF Definition of terms and units for the Instrumentation Data provided in 3.2 and 3.3. Open resource
3.3 UFS-3 LRB-AZ-1201 Instrumentation Data.csv CSV These data include the pH, oxidation-reduction potention (ORP, mv), pond water temperature (C), pond conductivity (ms.cm), dissolved oxygen concentration (DO, mg/L), dissolved oxygen saturation (%), salinity (g/L), and photosynthetically active radiation (PAR, umol/m2/s). The PAR sensor was connected to only one of the ponds. Open resource
3.2 UFS-3 K32 Instrumentation Data.csv CSV These data include the pH, oxidation-reduction potention (ORP, mv), pond water temperature (C), pond conductivity (ms.cm), dissolved oxygen concentration (DO, mg/L), dissolved oxygen saturation (%), salinity (g/L), and photosynthetically active radiation (PAR, umol/m2/s). The PAR sensor was connected to only one of the ponds. Open resource
1.1 Pond Operation and Harvest Summary Data Definitions.pdf PDF Definition of terms and units for the Pond Operation Data provided in 1.2 and 1.3. Open resource
1.3 UFS-3 LRB-AZ-1201 Pond Operation and Harvest Summary Data.csv CSV These data include the date, strain, batch and source ID, pond treatment and depth (cm), harvest number and time between harvests, harvest volume (L), amount (g), ash-free dry weight (AFDW; g/L) at the time of harvest, and an indication of a pond crash, operator comments, measured pond depth (cm), pH, salinity, pond water temperature (C), nitrogen concentration (ppm as N), phosphorus concentration (ppm as P), N:P ratio (molar), the sample ID and tracking ID of a physical sample (if taken), the algae concentration (g/L) as dry weight and ash-free dry weight (AFDW), the ash content of the algal biomass (%), and the optical density at 680nm and 750nm (OD750). Also supplied are calculated metrics of evaporation rate (cm/day), total N (NO3 or NH4) supplied (g), total N utilized (g), N efficiency (%), N demand (g N/g AFDW), total P supplied (g), total P utilized (g), P efficiency (%), P demand (g P/g AFDW), photosynthetic biomass production (mg AFDW/mol PAR (photosynthetically active radiation) input and mg AFDW/kW GLE (global light energy) input). Open resource
1.2 UFS-3 KA32 Pond Operation and Harvest Summary Data.csv CSV These data include the date, strain, batch and source ID, pond treatment and depth (cm), harvest number and time between harvests, harvest volume (L), amount (g), ash-free dry weight (AFDW; g/L) at the time of harvest, and an indication of a pond crash, operator comments, measured pond depth (cm), pH, salinity, pond water temperature (C), nitrogen concentration (ppm as N), phosphorus concentration (ppm as P), N:P ratio (molar), the sample ID and tracking ID of a physical sample (if taken), the algae concentration (g/L) as dry weight and ash-free dry weight (AFDW), the ash content of the algal biomass (%), and the optical density at 680nm and 750nm (OD750). Also supplied are calculated metrics of evaporation rate (cm/day), total N (NO3 or NH4) supplied (g), total N utilized (g), N efficiency (%), N demand (g N/g AFDW), total P supplied (g), total P utilized (g), P efficiency (%), P demand (g P/g AFDW), photosynthetic biomass production (mg AFDW/mol PAR (photosynthetically active radiation) input and mg AFDW/kW GLE (global light energy) input). Open resource
4.4 Daily Averaged Weather Data Definitions.pdf PDF Definition of terms and units for the Daily Averaged Weather Data provided in 4.5 and 4.6. Open resource
4.6 UFS-3 LRB-AZ-1201 Daily Averaged Weather Data.csv CSV These data include air temperature (C), relative humidity (%RH), Global Light Intensity (W/m2/s), daily precipitation (cm), wind speed (km/hr), and wind direction (degrees). Open resource
4.5 UFS-3 K32 Daily Averaged Weather Data.csv CSV These data include air temperature (C), relative humidity (%RH), Global Light Intensity (W/m2/s), daily precipitation (cm), wind speed (km/hr), and wind direction (degrees). Open resource
4.2 UFS-3 KA32 Complete KA32 Weather Data.csv CSV These data include air temperature (C), relative humidity (%RH), Global Light Intensity (W/m2/s), daily precipitation (cm), wind speed (km/hr), and wind direction (degrees). Open resource
4.1 Weather Data Definitions.pdf PDF Definition of terms and units for the Weather Data provided in 4.2 and 4.3. Open resource
4.3 UFS-3 LRB-AZ-1201 Complete Weather Data.csv CSV These data include air temperature (C), relative humidity (%RH), Global Light Intensity (W/m2/s), daily precipitation (cm), wind speed (km/hr), and wind direction (degrees). Open resource
0.3 ATP3 UFS Journal Article Unknown Journal article about the UFS studies providing detailed information about the experimental design, methods, and data collected. Open resource
0.2 Report on the Use of ATP3 UFS Data HTML Official report providing information on the use of the cultivation data from the ATP3 UFS experiments. Open resource
0.1 ATP3 OpenEI Wiki Page HTML ATP3 OpenEI Wiki page providing information about the Algae Testbed Public-Private Partnership Consortium and the Unified Field Study Data. Open resource
0.0 UFS-3 Experiment OpenEI Wiki Page HTML OpenEI Wiki page detailing the UFS-3 experiment, including experimental protocols and descriptions of each data resource. Open resource

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