Transcriptomic analysis of spleens from mice subjected to chronic low-dose radiation hindlimb unloading or a combination of both
Description
The purpose of this study was to evaluate transcriptional changes in mouse spleens using a ground-based model for spaceflight. This model includes prolonged unloading and low-dose irradiation. Low-dose-rate gamma-radiation was delivered to 6-month old female C57BL/6J mice using 57Co plates (0.04 Gy) to simulate the radiation environment of spaceflight. Anti-orthostatic tail suspension was used to model the unloading fluid shift and physiological stress aspects of the microgravity component of spaceflight. Mice were hindlimb suspended and/or irradiated for 21 days. Mice were euthanized and spleens collected 7 days following treatment. RNA sequencing data was generated to assess transcriptional changes in these spleens.
Resources
| Name |
Format |
Description |
Link |
|
21 |
GeneLab Study Page |
https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-211 |
Tags
- sample-collection
- ionizing-radiation
- nucleic-acid-extraction
- data-transformation
- treatment-protocol
- spike-in-protocol
- library-construction
- nucleic-acid-sequencing
- hindlimb-unloading
- genelab-rnaseq-data-processing-protocol