Transcription profiling of activated human T cells induced by microgravity to identify apoptotic genes and other immune response genes

Description

The purpose of this study was to search for microgravity-sensitive genes specifically for apoptotic genes influenced by the microgravity environment and other genes related to immune response. Experiment Overall Design: Two-group design with paired samples i.e. one 1G and one MMG culture came from the same donor. Therefore 6 samples came from 3 different donors. Experiment Overall Design: Donor 1 :GSM96146,GSM96147 Experiment Overall Design: Donor 2: GSM96148 GSM96149 Experiment Overall Design: Donor 3: GSM96150,GSM96151 Experiment Overall Design: Total RNA was submitted to and then labeled hybridized and data generated by the Baylor College Medicine Microarray Core Facility (333E One Baylor Plaza Houston TX 77030).

Resources

Name Format Description Link
21 GeneLab Study Page https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-5

Tags

  • scan-protocol
  • labeling-protocol
  • data-processing-protocol
  • sample-collection-protocol
  • hybridization-protocol
  • simulated-microgravity
  • nucleic-acid-extraction-protocol

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