The Heat-Inducible Transcription Factor HsfA2 Enhances Anoxia Tolerance in Arabidopsis
Description
Anoxia induces several heat shock proteins and a heat pre-treatment can acclimatize Arabidopsis seedlings to a subsequent anoxic treatment. In this work we analyzed the response of Arabidopsis seedlings to anoxia heat and a combined heat+anoxia stress. A significant overlapping between the anoxic and heat shock responses has been observed by whole-genome microarray analysis. Overall design: We treated Arabidopsis seedling 4-days old dark germinated with: Control (23C dark liquid Murashige-Skoog medium containing 30mM sucrose). Heat-treated (38C for 90 minutes dark liquid Murashige-Skoog medium containing 30mM sucrose). Anoxia-treated (23C under anoxia for 6h dark liquid Murashige-Skoog medium containing 30mM sucrose). Combined heat+Anoxia-treatment (23C treated at 38C for 90 min and thereafter under anoxia for 6h dark liquid Murashige-Skoog medium containing 30mM sucrose). Two biological replicates for each condition.
Resources
| Name |
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Description |
Link |
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21 |
GeneLab Study Page |
https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-282 |
Tags
- normalization-data-transformation
- data-collection
- treatment-protocol
- nucleic-acid-hybridization
- rna-extraction
- labeling
- growth-protocol
- treatment