Supplementary data for "Distributions of fitness effects for amino acid changes from high-throughout mutagenesis experiments" (McCandlish and Stoltzfus, 2018)

Description

McCandlish and Stoltzfus gathered data from deep mutational scanning experiments on 12 proteins, comprising 56641 distinct amino acid replacement mutations. By converting fitnesses to within-study quantiles, they combined results from all studies to draw general conclusions about distributions of fitness effects for the 380 different types of possible amino acid changes in proteins. They found that most replacements are neither conservative nor radical, but barely different from the background distribution. The shapes of these distributions can be approximated by a maximum-entropy model with only 1 parameter. This data package makes it possible to reproduce the main calculations used by Stoltzfus and McCandlish. The data also may be useful to researchers carrying out meta-analyses of mutation-scanning experiments or DFE experiments.

Resources

Name Format Description Link
47 https://data.nist.gov/od/ds/762AD4DA63D05927E05324570681D36C1970/ms2018_calculations.nb.sha256
49 all_obs.tsv https://data.nist.gov/od/ds/762AD4DA63D05927E05324570681D36C1970/all_obs.tsv
47 https://data.nist.gov/od/ds/762AD4DA63D05927E05324570681D36C1970/raw_obs.tsv.sha256
47 README.md https://data.nist.gov/od/ds/762AD4DA63D05927E05324570681D36C1970/README.md
0 https://doi.org/10.18434/M31970
47 https://data.nist.gov/od/ds/762AD4DA63D05927E05324570681D36C1970/figure_s1.pdf.sha256
29 ms2018_calculations.nb https://data.nist.gov/od/ds/762AD4DA63D05927E05324570681D36C1970/ms2018_calculations.nb
33 figure_s1.pdf https://data.nist.gov/od/ds/762AD4DA63D05927E05324570681D36C1970/figure_s1.pdf
47 https://data.nist.gov/od/ds/762AD4DA63D05927E05324570681D36C1970/all_obs.tsv.sha256
49 raw_obs.tsv https://data.nist.gov/od/ds/762AD4DA63D05927E05324570681D36C1970/raw_obs.tsv
47 https://data.nist.gov/od/ds/762AD4DA63D05927E05324570681D36C1970/README.md.sha256

Tags

  • fitness
  • deep-mutational-scanning
  • mutation
  • protein

Topics

Categories