STS-135: Mouse Liver Transcriptomics using RNA-Seq

Description

Female C57BL/6CR mice were flown onboard STS-135 for 13 days and returned to Earth for analysis. Livers were collected within 3-4 hours of landing and snap frozen in liquid nitrogen. Liver tissue samples that were used for microarray analysis for GLDS-25 were provided to GeneLab. GeneLab extracted RNA added ERCC control spike-in to the samples and performed RNA-Seq analysis.

Resources

Name Format Description Link
21 GeneLab Study Page https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-173

Tags

  • sample-collection
  • radiation-dosimetry
  • cosmic-radiation
  • spike-in-control
  • space-flight
  • nucleic-acid-extraction
  • data-transformation
  • library-construction
  • nucleic-acid-sequencing
  • genelab-rnaseq-data-processing-protocol
  • radiation-dosimetry-protocol

Topics

Categories