Description
This study aimed to optimize a previously used indirect processing protocol, which included a series of washing and centrifugation steps. Optimization of the protocol included: identifying an ideal extraction diluent and evaluating variation in the number of wash steps, variation in the initial centrifugation speed, sonication and shaking mechanisms. The optimized protocol was demonstrated at two laboratories in order to evaluate the recovery of spores from loamy and sandy soils. Data supplied are statistical data and were used to help support conclusions and tables in the journal article.
This dataset is associated with the following publication:
Silvestri, E., D. Feldhake, D. Griffin, J. Lisle, T. Nichols, S. Shah, A. Pemberton, and F. Schaefer. Optimization of a Sample Processing Protocol for Recovery of Bacillus anthracis Spores from Soil [HS7.52.02 - 514]. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, USA, 130: 6-13, (2016).
Resources
| Name | Format | Description | Link |
|---|---|---|---|
| ANNOVA comparison tables sandy and loamy soils.xlsx | XLS | ANNOVA%20comparison%20tables%20sandy%20and%20loamy%20soils.xlsx | Open resource |
| Data Back Log10 Transformed and geometic means Summary Stats LV SV.xlsx | XLS | Data%20Back%20Log10%20Transformed%20%20and%20geometic%20means%20Summary%20Stats%20LV%20%20SV.xlsx | Open resource |
| Descriptive Statistics for raw and log10 data with data dictionary 121815.xlsx | XLS | Descriptive%20Statistics%20for%20raw%20and%20log10%20data%20with%20data%20dictionary%20121815.xlsx | Open resource |
| Laboratory Comparison Data 121815.xlsx | XLS | Laboratory%20Comparison%20Data%20121815.xlsx | Open resource |
| Percent Recovery Efficiency data with data dictionary.xlsx | XLS | Percent%20Recovery%20Efficiency%20data%20with%20data%20dictionary.xlsx | Open resource |
| Table 1_Bacillus anthracis Sterne Spore Recoveries_metadata.docx | Doc | Table%201_Bacillus%20anthracis%20Sterne%20Spore%20Recoveries_metadata.docx | Open resource |
| Table 2_Average Spore Recovery for Mechanical Shaking versus Manual_metadata.docx | Doc | Table%202_Average%20Spore%20Recovery%20for%20Mechanical%20Shaking%20versus%20Manual_metadata.docx | Open resource |