Soil processing method journal article supporting data

Description

This study aimed to optimize a previously used indirect processing protocol, which included a series of washing and centrifugation steps. Optimization of the protocol included: identifying an ideal extraction diluent and evaluating variation in the number of wash steps, variation in the initial centrifugation speed, sonication and shaking mechanisms. The optimized protocol was demonstrated at two laboratories in order to evaluate the recovery of spores from loamy and sandy soils. Data supplied are statistical data and were used to help support conclusions and tables in the journal article. This dataset is associated with the following publication: Silvestri, E., D. Feldhake, D. Griffin, J. Lisle, T. Nichols, S. Shah, A. Pemberton, and F. Schaefer. Optimization of a Sample Processing Protocol for Recovery of Bacillus anthracis Spores from Soil [HS7.52.02 - 514]. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, USA, 130: 6-13, (2016).

Resources

Name Format Description Link
53 ANNOVA%20comparison%20tables%20sandy%20and%20loamy%20soils.xlsx https://pasteur.epa.gov/uploads/184/ANNOVA%20comparison%20tables%20sandy%20and%20loamy%20soils.xlsx
53 Laboratory%20Comparison%20Data%20121815.xlsx https://pasteur.epa.gov/uploads/184/Laboratory%20Comparison%20Data%20121815.xlsx
10 Table%202_Average%20Spore%20Recovery%20for%20Mechanical%20Shaking%20versus%20Manual_metadata.docx https://pasteur.epa.gov/uploads/184/Table%202_Average%20Spore%20Recovery%20for%20Mechanical%20Shaking%20versus%20Manual_metadata.docx
53 Percent%20Recovery%20Efficiency%20data%20with%20data%20dictionary.xlsx https://pasteur.epa.gov/uploads/184/Percent%20Recovery%20Efficiency%20data%20with%20data%20dictionary.xlsx
53 Descriptive%20Statistics%20for%20raw%20and%20log10%20data%20with%20data%20dictionary%20121815.xlsx https://pasteur.epa.gov/uploads/184/Descriptive%20Statistics%20for%20raw%20and%20log10%20data%20with%20data%20dictionary%20121815.xlsx
10 Table%201_Bacillus%20anthracis%20Sterne%20Spore%20Recoveries_metadata.docx https://pasteur.epa.gov/uploads/184/Table%201_Bacillus%20anthracis%20Sterne%20Spore%20Recoveries_metadata.docx
53 Data%20Back%20Log10%20Transformed%20%20and%20geometic%20means%20Summary%20Stats%20LV%20%20SV.xlsx https://pasteur.epa.gov/uploads/184/Data%20Back%20Log10%20Transformed%20%20and%20geometic%20means%20Summary%20Stats%20LV%20%20SV.xlsx

Tags

  • sand
  • indirect-processing
  • spores
  • bacillus-anthracis
  • soil
  • loam

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