Sablefish GI microbiome & histology - Incorporating Gastrointestinal Microbiome Analysis into Fish Nutritional Assessments

Description

Sustainable culture of sablefish (Anoplopoma fimbria) is an active research area at NWFSC, with a primary objective of rearing a human food source under healthy, economic, and sustainable conditions. Development of sustainable feed for juvenile fish through replacement of marine fish oils is key for economic and ecological viability of sablefish culture. This project intends to analyze the GI microbiome of juvenile sablefish, comparing three feeds (corn oil, linseed oil, BioOregon BioBrood) used in a National Marine Aquaculture Initiative (NMAI)-funded project examining the effects of substitute lipids on growth, production composition, and lipid bioconversion. Community DNA from GI mucus and luminal contents will be extracted, quantified, and quality checked. DNA will be submitted for analysis by a microbial phylogenetic microarray bearing over a million probes for classifying more than 59,000 bacterial taxa. Second Genome, a company with exclusive licensing for this phylogenetic microarray, will conduct the the analysis for classification, relative abundance, & ordination. Complementary to microbiome analysis, morphological changes in gastrointestinal tissues will be assessed for each fish by light microscopic histology (collaboration with Mark Myers). Although this is a stand-alone project, the objective is for the microbiome approach to become an integral component of finfish nutrition research. Raw & refined classification & abundance data from array analysis & histology analysis.

Resources

Name Format Description Link
0 These data were derived from a feeding study with juvenile sablefish, comparing plant-based feeds (flax, corn) with fish-based feed (reference). Intestinal microbiomes were determined by hybridization of a gastronintesntial DNA microarray. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/comparative_bacterial_and_archaeal_gastrointestinal_abundances_from_sablefish_on_different_diets
0 These data were derived from a feeding study with juvenile sablefish, comparing plant-based feeds (flax, corn) with fish-based feed (reference). Intestinal microbiomes were determined by hybridization of a gastronintesntial DNA microarray. https://www.webapps.nwfsc.noaa.gov/apex/parr/comparative_bacterial_and_archaeal_gastrointestinal_abundances_from_sablefish_on_different_diets/data/page/
0 View the complete metadata record on InPort for more information about this dataset. https://www.fisheries.noaa.gov/inport/item/18020
0 NWFSC Dataset Information page. Raw & refined classification & abundance data from array analysis & histology analysis https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/datasets/dataset/7390
0 The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. https://www.earthdata.nasa.gov/learn/find-data/idn/gcmd-keywords
33 NOAA Data Management Plan for this record on InPort. https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/nwfsc/dmp/pdf/18020.pdf

Tags

  • microbiome
  • animal and plant collection device
  • aquaculture
  • instrument not applicable
  • migrated from resource enhancement and utilization technologies division
  • northwest fisheries science center
  • nwfsc montlake
  • doc/noaa/nmfs/nwfsc
  • national marine fisheries service
  • noaa. u.s. department of commerce
  • montlake
  • fish diet
  • sablefish
  • alternative feeds

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