RGTM 10169

Description

NIST developed a reference material consisting of synthetic fragments of the SARS-CoV-2 virus RNA, which is the target of molecular diagnostic tests. These RNA fragments can assist in the development and validation of RT-qPCR assays for the detection SARS-CoV-2. The RNA fragments are characterized for concentration using digital PCR methods, may be used to assess limits of detection for SARS-CoV-2 assays, and may calibrate other in-house or commercial SARS-CoV-2 controls. This dataset includes data used for RTGM 10169 SARs-Cov-2 Research Grade Test Material validation. The Illumina and ONT sequence data were used to verify the construct sequence.

Resources

Name Format Description Link
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_c1.vcf.gz.tbi.sha256
57 ill_c1_R1_fastqc.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/fq/ill_c1_R1_fastqc.zip
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/fq/ill_c1_R1_fastqc.zip.sha256
57 ill_c1_R2_fastqc.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/fq/ill_c1_R2_fastqc.zip
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/fq/ill_c1_R2_fastqc.zip.sha256
57 ill_c2_R1_fastqc.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/fq/ill_c2_R1_fastqc.zip
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc.log.sha256
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_citations.txt.sha256
5 ont.bam.bai https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont.bam.bai
0 information related to Research Grade Test Material (RGTM) 10169 https://github.com/usnistgov/RGTM10169
47 ill_c1.txt https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/bam/ill_c1.txt
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/bam/ill_c1.txt.sha256
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/bam/ont.txt.sha256
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_general_stats.txt.sha256
47 multiqc_citations.txt https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_citations.txt
47 multiqc_fastqc.txt https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_fastqc.txt
47 multiqc_samtools_stats.txt https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_samtools_stats.txt
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_samtools_stats.txt.sha256
47 multiqc_sources.txt https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_sources.txt
19 c1_R1_001.fastq.gz https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/c1_R1_001.fastq.gz
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/c1_R1_001.fastq.gz.sha256
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/c1_R2_001.fastq.gz.sha256
19 c2_R1_001.fastq.gz https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/c2_R1_001.fastq.gz
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/c2_R1_001.fastq.gz.sha256
19 c2_R2_001.fastq.gz https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/c2_R2_001.fastq.gz
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill.bam.sha256
5 ill.bam.bai https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill.bam.bai
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill.bam.bai.sha256
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_c1.vcf.gz.sha256
5 ill_c1.vcf.gz.tbi https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_c1.vcf.gz.tbi
19 ill_c2.vcf.gz https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_c2.vcf.gz
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_c2.vcf.gz.sha256
5 ill_c2.vcf.gz.tbi https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_c2.vcf.gz.tbi
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_c2.vcf.gz.tbi.sha256
47 ill_depth.txt https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_depth.txt
47 md5sum_list.txt https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/md5sum_list.txt
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/md5sum_list.txt.sha256
5 ont.bam https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont.bam
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont.bam.sha256
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont.bam.bai.sha256
19 ont.fastq.gz https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont.fastq.gz
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont.fastq.gz.sha256
47 ont_depth.txt https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont_depth.txt
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont_depth.txt.sha256
5 construct1_w_flankings.fa https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/construct1_w_flankings.fa
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/construct1_w_flankings.fa.sha256
5 annotations.bed https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/annotations.bed
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/annotations.bed.sha256
5 construct2_w_flankings.fa https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/construct2_w_flankings.fa
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/construct2_w_flankings.fa.sha256
5 constructs_w_flankings.fa https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/constructs_w_flankings.fa
5 primers.bed https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/primers.bed
5 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/sars-cov-2-RGTM.genome
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/sars-cov-2-RGTM.genome.sha256
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/README.md.sha256
47 multiqc.log https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc.log
47 README.md https://data.nist.gov/od/ds/ark:/88434/mds2-2714/README.md
57 This zip file contains multiqc.html https://data.nist.gov/od/ds/mds2-2714/multiqc%20html.zip
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/fq/ill_c2_R1_fastqc.zip.sha256
57 ill_c2_R2_fastqc.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/fq/ill_c2_R2_fastqc.zip
23 multiqc_data.json https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_data.json
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_data.json.sha256
19 c1_R2_001.fastq.gz https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/c1_R2_001.fastq.gz
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_depth.txt.sha256
47 ont_guppy_sequencing_summary.txt https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont_guppy_sequencing_summary.txt
57 This file contains the ont_pycoqc.html file https://data.nist.gov/od/ds/mds2-2714/ont%20pycoqc%20html.zip
47 ill_c2.txt https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/bam/ill_c2.txt
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/bam/ill_c2.txt.sha256
47 ont.txt https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/bam/ont.txt
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_sources.txt.sha256
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/c2_R2_001.fastq.gz.sha256
5 ill.bam https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill.bam
19 ill_c1.vcf.gz https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/ill_c1.vcf.gz
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/fq/ill_c2_R2_fastqc.zip.sha256
5 md5.chk https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/md5.chk
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ill/md5.chk.sha256
47 multiqc_general_stats.txt https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_general_stats.txt
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/analyses/qc/multiqc_data/multiqc_fastqc.txt.sha256
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/constructs_w_flankings.fa.sha256
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/ont_guppy_sequencing_summary.txt.sha256
19 fast5s.tar.gz https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/fast5s.tar.gz
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ont/fast5s.tar.gz.sha256
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2714/ref/primers.bed.sha256

Tags

  • pcr
  • reference-material
  • sequencing
  • srm
  • biosciences-and-health
  • digital-pcr
  • virus
  • dna

Topics

Categories