Description
NIST developed a reference material consisting of synthetic fragments of the SARS-CoV-2 virus RNA, which is the target of molecular diagnostic tests. These RNA fragments can assist in the development and validation of RT-qPCR assays for the detection SARS-CoV-2. The RNA fragments are characterized for concentration using digital PCR methods, may be used to assess limits of detection for SARS-CoV-2 assays, and may calibrate other in-house or commercial SARS-CoV-2 controls. This dataset includes data used for RTGM 10169 SARs-Cov-2 Research Grade Test Material validation. The Illumina and ONT sequence data were used to verify the construct sequence.
Resources
| Name | Format | Description | Link |
|---|---|---|---|
| Text File | Text | ill_c1.txt | Open resource |
| Text File | Text | Open resource | |
| Text File | Text | ill_c2.txt | Open resource |
| Text File | Text | Open resource | |
| Text File | Text | ont.txt | Open resource |
| Text File | Text | Open resource | |
| Zip File | ZIP | ill_c1_R1_fastqc.zip | Open resource |
| Text File | Text | Open resource | |
| Zip File | ZIP | ill_c1_R2_fastqc.zip | Open resource |
| Text File | Text | Open resource | |
| Zip File | ZIP | ill_c2_R1_fastqc.zip | Open resource |
| Text File | Text | Open resource | |
| Zip File | ZIP | ill_c2_R2_fastqc.zip | Open resource |
| Text File | Text | Open resource | |
| Text File | Text | multiqc_citations.txt | Open resource |
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| JSON File | JSON | multiqc_data.json | Open resource |
| Text File | Text | Open resource | |
| Text File | Text | multiqc_fastqc.txt | Open resource |
| Text File | Text | Open resource | |
| Text File | Text | multiqc_general_stats.txt | Open resource |
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| Text File | Text | multiqc.log | Open resource |
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| Text File | Text | multiqc_samtools_stats.txt | Open resource |
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| Text File | Text | multiqc_sources.txt | Open resource |
| Text File | Text | Open resource | |
| Web Resource | G-Zipped | c1_R1_001.fastq.gz | Open resource |
| Text File | Text | Open resource | |
| Web Resource | G-Zipped | c1_R2_001.fastq.gz | Open resource |
| Text File | Text | Open resource | |
| Web Resource | G-Zipped | c2_R1_001.fastq.gz | Open resource |
| Text File | Text | Open resource | |
| Web Resource | G-Zipped | c2_R2_001.fastq.gz | Open resource |
| Text File | Text | Open resource | |
| Web Resource | Binary | ill.bam | Open resource |
| Web Resource | Binary | ill.bam.bai | Open resource |
| Text File | Text | Open resource | |
| Text File | Text | Open resource | |
| Web Resource | G-Zipped | ill_c1.vcf.gz | Open resource |
| Text File | Text | Open resource | |
| Web Resource | Binary | ill_c1.vcf.gz.tbi | Open resource |
| Text File | Text | Open resource | |
| Web Resource | G-Zipped | ill_c2.vcf.gz | Open resource |
| Text File | Text | Open resource | |
| Web Resource | Binary | ill_c2.vcf.gz.tbi | Open resource |
| Text File | Text | Open resource | |
| Text File | Text | ill_depth.txt | Open resource |
| Text File | Text | Open resource | |
| Web Resource | Binary | md5.chk | Open resource |
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| Text File | Text | md5sum_list.txt | Open resource |
| Text File | Text | Open resource | |
| Web Resource | G-Zipped | fast5s.tar.gz | Open resource |
| Text File | Text | Open resource | |
| Web Resource | Binary | ont.bam | Open resource |
| Web Resource | Binary | ont.bam.bai | Open resource |
| Text File | Text | Open resource | |
| Text File | Text | Open resource | |
| Text File | Text | ont_depth.txt | Open resource |
| Text File | Text | Open resource | |
| Web Resource | G-Zipped | ont.fastq.gz | Open resource |
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| Text File | Text | ont_guppy_sequencing_summary.txt | Open resource |
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| Text File | Text | README.md | Open resource |
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| Web Resource | Binary | annotations.bed | Open resource |
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| Web Resource | Binary | construct1_w_flankings.fa | Open resource |
| Text File | Text | Open resource | |
| Web Resource | Binary | construct2_w_flankings.fa | Open resource |
| Text File | Text | Open resource | |
| Web Resource | Binary | constructs_w_flankings.fa | Open resource |
| Text File | Text | Open resource | |
| Web Resource | Binary | primers.bed | Open resource |
| Text File | Text | Open resource | |
| Web Resource | Binary | Open resource | |
| Text File | Text | Open resource | |
| multiqc.html | ZIP | This zip file contains multiqc.html | Open resource |
| ont_pycoqc.html | ZIP | This file contains the ont_pycoqc.html file | Open resource |
| Research Grade Test Material (RGTM) 10169 | Unknown | information related to Research Grade Test Material (RGTM) 10169 | Open resource |