Description
Background
Peroxynitrite is increasingly proposed as a contributor to defence system in marine bivalve. It can be formed by combinaison of superoxide and nitric oxide, and can react with tyrosine residues of proteins giving rise to 3-nitrotyrosine.
Results
The present article describes a competitive ELISA for the measurement of 3-nitrotyrosine contents of plasma proteins from marine bivalves by means of a monoclonal anti 3-nitrotyrosine antibody mouse IgG.
Conclusions
This assay is sensitive enough to determine the amounts of 3-nitrotyrosine in plasma proteins from one animal only.
Using the C-ELISA, we have shown that the phagocytosis of zymosan particles increased the 3-nitrotyrosine levels of plasma proteins from mussel M. galloprovincialis and oyster C. gigas 5.8 and 7.5 times respectively.
Resources
| Name | Format | Description | Link |
|---|---|---|---|
| Official Government Data Source | HTML | Visit the original government dataset for complete information, documentation, and data access. | Open resource |