Reporting the limits of detection (LOD) and quantification (LOQ) for environmental DNA assays: Data

Description

This data set was collected to provide examples and aid in developing a standardized way of determining LOD and LOQ for eDNA assays and has 3 data files. GEDWG_LOD_DATA3.csv is raw qPCR data from multiple labs running multiple standards of known concentration for eDNA assays they regularly use. Comparison-Data.csv is the merged data output from running a generic LOD/LOQ calculator script multiple times with different LOD model settings. The generic LOD/LOQ calculator script is available at: https://github.com/cmerkes/qPCR_LOD_Calc, and details about the multiple settings used are commented in the analysis script available at: https://github.com/cmerkes/LOD_Analysis

Resources

Name Format Description Link
55 Landing page for access to the data https://doi.org/10.5066/P9AKHU1R
55 The metadata original format https://data.usgs.gov/datacatalog/metadata/USGS.5d36fbfce4b01d82ce8a6cbf.xml

Tags

  • pcr
  • ontario-ministry-of-natural-resources-and-forestry-2140-east-bank-drive-peterborough-ontario-k9
  • national-wildlife-research-center-4101-laporte-avenue-fort-collins-colorado-80521
  • whitney-genetics-laboratory-555-lester-avenue-onalaska-wisconsin-54650
  • usgs-5d36fbfce4b01d82ce8a6cbf
  • biota
  • school-of-the-environment-washington-state-university-100-dairy-road-pullman-washington-99164
  • engineers-research-and-development-center-3909-halls-ferry-road-vicksburg-mississippi-39180
  • environmental-dna
  • edna
  • upper-midwest-environmental-sciences-center-2630-fanta-reed-road-la-crosse-wisconsin-54603
  • invasive-species
  • department-of-biochemistry-and-microbiology-university-of-victoria-3800-finnerty-road-victoria-
  • detection
  • columbia-environmental-research-center-4200-e-new-haven-road-columbia-missouri-65201

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