Rare Earth Desorption Test with Citrate and Bicarbonate
Description
LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min.
Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.
Resources
| Name |
Format |
Description |
Link |
|
53 |
Excel file detailing results of the bicarbonate and citrate desorption experiments. Plots of results included. |
https://gdr.openei.org/files/965/GTO_data_deposit_092017_Desorption.xlsx |
Tags
- brine
- resource-recovery
- lanthanide-binding-tag
- citrate
- ree
- lbt
- centrifuge
- desorption
- bicarbonate
- rare-earth
- geothermal
- adsorption
- energy