Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, evaluation of assays, 2019-2022

This data release documents results of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia, western mosquito fish and guppies in pools with known fish populations and in pre and post-treatment samples from pools that were treated with carbon dioxide gas. These assays provide new tools for resource managers to monitor effectiveness of management efforts to remove invasive fish from anchialine pools in Hawaii and to also survey pools for presence and absence of invasive fish. The lab work was conducted during 2019-2022.