Proteomic Signatures of Corals from Thermodynamic Reefs (NCEI Accession 0216077)

Description

In this work, I took a protein profiling approach known as iTRAQ approach to characterize and quantify the proteins synthesized by 12 experimental coral samples. Specifically, corals were collected from an upwelling site in the Luzon Strait ("Houbihu"; 21°56’18.01’’N, 120°44’45.54’’E) and a non-upwelling "control" site in the nearby Taiwan Strait ("Houwan"; 22°01'23.30"N, 120°41'18.29"E) and exposed to either highly variable (upwelling-simulating) or stable temperatures in the laboratory for seven days prior to sacrificing for protein analysis (the "Seriatopora hystrix variable temperature study" [SHVTS]). In this submission, I have uploaded the proofs of the manuscript describing the proteomic data (Mayfield in press Microorganisms), two RAW files (SHVTS-A & SHVTS-B for iTRAQ batches A & B, respectively), two MZML (open-access mass spectrometry data) files (SHVTS-A.mzml & SHVTS-B.mzml), two MZID files (SHVTS-A.mzid & SHVTS-B.mzid), and a tab-delimited file that describes which samples were labeled with which iTRAQ tag ("Mayfield online supplemental data file"). I have also uploaded the supplemental material that accompanies the Mayfield (in press) article since that is what readers interested in the specifics of the proteomic analysis pipeline should consult. The peak data were queried against a conceptually translated S. hystrix-Cladocopium dinoflagellate transcriptome described in Mayfield et al. (2016 Mol Ecol) and found here: http://symbiont.iis.sinica.edu.tw/s_hystrix/static/html/#stat. Please note that all proteomic data represent a mix of multiple organisms (namely the anthozoan host and the dinoflagellate endosymbionts that inhabit their gastrodermal cells). All data analysis was conducted with Proteome Discoverer (Thermo-Fisher Scientific).

Resources

Name Format Description Link
0 Institution web page https://www.aoml.noaa.gov/
0 Institution web page https://www.aoml.noaa.gov/
0 These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0159/0216077/
0 Global Change Master Directory (GCMD). 2023. GCMD Keywords, Version 16. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Institution web page https://www.aoml.noaa.gov/
0 Navigate directly to the URL for a descriptive web page with download links. https://www.ncei.noaa.gov/archive/accession/0216077
0 Institution web page http://www.miami.edu/
0 Mayfield, A.B., Chan, P.H., Putnam, H.P., Chen, C.S., Fan, T.Y. 2012. The effects of a variable temperature regime on the physiology of the reef-building coral Seriatopora hystrix: results from a laboratory-based reciprocal transplant. Journal of Experimental Biology 215: 4183-4195. https://doi.org/10.1242/jeb.071688 https://doi.org/10.1242/jeb.071688
0 Global Change Master Directory (GCMD). 2023. GCMD Keywords, Version 16. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Navigate directly to the URL for a descriptive web page with download links. https://www.ncei.noaa.gov/archive/accession/oas/216077
0 Navigate directly to the URL for data access and direct download. https://www.ncei.noaa.gov/archive/accession/download/216077
0 Information for contacts at NCEI. https://www.ncei.noaa.gov/contact
0 Institution web page http://www.miami.edu/
0 Institution web page http://www.miami.edu/
0 Institution web page https://cimas.earth.miami.edu/
0 Information for contacts at NCEI. https://www.ncei.noaa.gov/contact
0 MassIVE Metadata Record https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=558adcff310b48279b6692dbe3ea41c0
0 NOAA Central Library: Journal Article https://doi.org/10.3390/microorganisms8081171
0 Institution web page https://library.noaa.gov/
0 Global Change Master Directory (GCMD). 2023. GCMD Keywords, Version 16. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2023. GCMD Keywords, Version 16. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Navigate directly to the URL for a descriptive web page with download links. https://www.ncei.noaa.gov/archive/accession/0216077
0 Navigate directly to the URL for a descriptive web page with download links. https://www.ncei.noaa.gov/archive/accession/oas/216077
0 Navigate directly to the URL for data access and direct download. https://www.ncei.noaa.gov/archive/accession/download/216077
0 These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0159/0216077/
0 Information for contacts at NCEI. https://www.ncei.noaa.gov/contact
0 Institution web page https://www.aoml.noaa.gov/
0 Institution web page https://www.aoml.noaa.gov/
0 Institution web page http://www.miami.edu/
0 Institution web page http://www.miami.edu/
0 Institution web page https://cimas.earth.miami.edu/
0 Institution web page https://www.aoml.noaa.gov/
0 Institution web page http://www.miami.edu/
0 Information for contacts at NCEI. https://www.ncei.noaa.gov/contact
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 MassIVE Metadata Record https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=558adcff310b48279b6692dbe3ea41c0
0 related journal article https://doi.org/10.1242/jeb.071688
0 NOAA Central Library: Journal Article https://doi.org/10.3390/microorganisms8081171
0 Navigate directly to the URL for a descriptive web page with download links. https://www.ncei.noaa.gov/archive/accession/0216077
0 Navigate directly to the URL for a descriptive web page with download links. https://www.ncei.noaa.gov/archive/accession/oas/216077
0 Navigate directly to the URL for data access and direct download. https://www.ncei.noaa.gov/archive/accession/download/216077
0 These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0159/0216077/
0 Information for contacts at NCEI. https://www.ncei.noaa.gov/contact
0 Institution web page https://www.aoml.noaa.gov/
0 Institution web page https://www.aoml.noaa.gov/
0 Institution web page http://www.miami.edu/
0 Institution web page http://www.miami.edu/
0 Institution web page https://cimas.earth.miami.edu/
0 Institution web page https://www.aoml.noaa.gov/
0 Institution web page http://www.miami.edu/
0 Information for contacts at NCEI. https://www.ncei.noaa.gov/contact
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 related journal article https://doi.org/10.1242/jeb.071688
0 NOAA Central Library: Journal Article https://doi.org/10.3390/microorganisms8081171
0 MassIVE Metadata Record https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=558adcff310b48279b6692dbe3ea41c0
0 Navigate directly to the URL for a descriptive web page with download links. https://www.ncei.noaa.gov/archive/accession/0216077
0 Navigate directly to the URL for a descriptive web page with download links. https://www.ncei.noaa.gov/archive/accession/oas/216077
0 Navigate directly to the URL for data access and direct download. https://www.ncei.noaa.gov/archive/accession/download/216077
0 These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0159/0216077/
0 Information for contacts at NCEI. https://www.ncei.noaa.gov/contact
0 Institution web page https://www.aoml.noaa.gov/
0 Institution web page https://www.aoml.noaa.gov/
0 Institution web page http://www.miami.edu/
0 Institution web page http://www.miami.edu/
0 Institution web page https://cimas.earth.miami.edu/
0 Institution web page https://www.aoml.noaa.gov/
0 Institution web page http://www.miami.edu/
0 Information for contacts at NCEI. https://www.ncei.noaa.gov/contact
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 MassIVE Metadata Record https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=558adcff310b48279b6692dbe3ea41c0
0 related journal article https://doi.org/10.1242/jeb.071688
0 NOAA Central Library: Journal Article https://doi.org/10.3390/microorganisms8081171
0 Navigate directly to the URL for a descriptive web page with download links. https://www.ncei.noaa.gov/archive/accession/0216077
0 Navigate directly to the URL for a descriptive web page with download links. https://www.ncei.noaa.gov/archive/accession/oas/216077
0 Navigate directly to the URL for data access and direct download. https://www.ncei.noaa.gov/archive/accession/download/216077
0 These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0159/0216077/
0 Information for contacts at NCEI. https://www.ncei.noaa.gov/contact
0 Institution web page https://www.aoml.noaa.gov/
0 Institution web page https://www.aoml.noaa.gov/
0 Institution web page http://www.miami.edu/
0 Institution web page http://www.miami.edu/
0 Institution web page https://cimas.earth.miami.edu/
0 Institution web page https://www.aoml.noaa.gov/
0 Institution web page http://www.miami.edu/
0 Information for contacts at NCEI. https://www.ncei.noaa.gov/contact
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 MassIVE Metadata Record https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=558adcff310b48279b6692dbe3ea41c0
0 related journal article https://doi.org/10.1242/jeb.071688
0 NOAA Central Library: Journal Article https://doi.org/10.3390/microorganisms8081171

Tags

  • molecular genetic analysis
  • belj6l
  • reef monitoring and assessment
  • corals
  • biosphere
  • proteomic analysis
  • oceans
  • numeric data sets
  • oceanography
  • biology
  • scleractinia (stony corals)
  • earth science
  • marine biology
  • ocean basin
  • coral
  • hengchung
  • taiwan
  • coastal processes
  • nanwan bay (21n120e0008)
  • zoology
  • coral reefs
  • pacific ocean
  • country/territory

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