Plasma Standards

Description

Digital droplet PCR fluorsence amplitude values from which plasmid copy concentrations of DNA standards were determined as described in D-EMMD-MEB-018-SOP-01and Journal article. Summary of stability testing results also provided. This dataset is associated with the following publication: Sivaganesan, M., M. Varma, S. Siefring, and R. Haugland. Quantification of plasmid DNA standards for U.S. EPA fecal indicator bacteria qPCR methods by droplet digital PCR analysis. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, USA, 152: 135-142, (2018).

Resources

Name Format Description Link
53 Batch2_Data_Dictionary.xlsx https://pasteur.epa.gov/uploads/10.23719/1500439/Batch2_Data_Dictionary.xlsx
53 Batch2_stability_summary.xlsx https://pasteur.epa.gov/uploads/10.23719/1500439/Batch2_stability_summary.xlsx
53 Batch2_Tube3_plasmid_amplitudes_summary.xlsx https://pasteur.epa.gov/uploads/10.23719/1500439/Batch2_Tube3_plasmid_amplitudes_summary.xlsx
53 Batch2_Tube2_plasmid_amplitudes_summary.xlsx https://pasteur.epa.gov/uploads/10.23719/1500439/Batch2_Tube2_plasmid_amplitudes_summary.xlsx
53 Batch2_Tube5_plasmid_amplitudes_summary.xlsx https://pasteur.epa.gov/uploads/10.23719/1500439/Batch2_Tube5_plasmid_amplitudes_summary.xlsx
53 Batch2_Tube1_plasmid_amplitudes_summary.xlsx https://pasteur.epa.gov/uploads/10.23719/1500439/Batch2_Tube1_plasmid_amplitudes_summary.xlsx
53 Batch2_Tube4_plasmid_amplitudes_summary.xlsx https://pasteur.epa.gov/uploads/10.23719/1500439/Batch2_Tube4_plasmid_amplitudes_summary.xlsx
53 Batch2_Tube1-5_Data_Dictionary.xlsx https://pasteur.epa.gov/uploads/10.23719/1500439/Batch2_Tube1-5_Data_Dictionary.xlsx

Tags

  • digital-droplet-pcr
  • qpcr
  • draft-method-c
  • epa-methods-1611-1
  • epa-methods-1609-1
  • dna-standards

Topics

Categories