Phenotypic Profiling of 6PPD, 6PPD-Quinone, and Structurally Diverse Antiozonants in RTgill-W1 Cells Using the Cell Painting Assay

Description

Methods S1–S2 detailing laboratory and data analysis methods; tables describing materials used (Table S1), assay wells excluded from analyses (Table S2), sourcing information for test chemicals (Table S3), cell culture media (Table S4) and assay reagents (Table S5), and descriptions and metadata for data files S1–S9 (PDF) File S1 containing the 2D chemical structures for all antiozonant compounds tested in this study (PDF) File S2 containing the list of features used for profile correlation analysis (XLSX) File S3 containing graphs of each cell viability and Cell Painting concentration–response curve for all tested chemicals in the study (PDF) File S4 containing the curve-fitting results for the cell viability assay (XLSX) File S5 containing the curve-fitting results for the Cell Painting assay (XLSX) File S6 containing a list of all features collected during Cell Painting and their metadata (XLSX) File S7 containing the normalized well-level values for the Cell Painting assay (XLSX) File S8 containing global Mahalanobis distance values for the Cell Painting assay, 1 per well (XLSX) File S9 containing category Mahalanobis distance values for the Cell Painting assay, 49 per well (XLSX). This dataset is associated with the following publication: Harris, F., M. Jankowski, D. Villeneuve, and J. Harrill. Phenotypic Profiling of 6PPD, 6PPD-quinone and Structurally Diverse Antiozonants in RTgill-W1 Cells Using the Cell Painting Assay. Environmental Science & Technology Letters. American Chemical Society, Washington, DC, USA, 12(6): 695-701, (2025).

Resources

Name Format Description Link
33 ez5c00327_si_002.pdf https://pasteur.epa.gov/uploads/10.23719/1532365/ez5c00327_si_002.pdf
53 ez5c00327_si_003.xlsx https://pasteur.epa.gov/uploads/10.23719/1532365/ez5c00327_si_003.xlsx
33 ez5c00327_si_004.pdf https://pasteur.epa.gov/uploads/10.23719/1532365/ez5c00327_si_004.pdf
53 ez5c00327_si_005.xlsx https://pasteur.epa.gov/uploads/10.23719/1532365/ez5c00327_si_005.xlsx
53 ez5c00327_si_006.xlsx https://pasteur.epa.gov/uploads/10.23719/1532365/ez5c00327_si_006.xlsx
53 ez5c00327_si_007.xlsx https://pasteur.epa.gov/uploads/10.23719/1532365/ez5c00327_si_007.xlsx
53 ez5c00327_si_008.xlsx https://pasteur.epa.gov/uploads/10.23719/1532365/ez5c00327_si_008.xlsx
53 ez5c00327_si_009.xlsx https://pasteur.epa.gov/uploads/10.23719/1532365/ez5c00327_si_009.xlsx
53 ez5c00327_si_010.xlsx https://pasteur.epa.gov/uploads/10.23719/1532365/ez5c00327_si_010.xlsx
33 ez5c00327_si_001.pdf https://pasteur.epa.gov/uploads/10.23719/1532365/ez5c00327_si_001.pdf

Tags

  • cell-painting
  • 6ppd-quinone
  • ecotoxicology
  • rainbow-trout
  • phenotypic-profiling
  • in-vitro-toxicology

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