Patent AT-E401415-T1: [Translated] DIRECT RT-PCR ON MICROTITER PLATES WITH IMMOBILIZED OLIGONUCLEOTIDE
Description
The entire process of reverse transcription-polymerase chain reaction (RT-PCR) is simplified by using oligonucleotide-immobilized microplates made of, e.g., polypropylene, to which oligonucleotides are securely immobilized and which can be subjected to thermal cycles of PCR. RT-PCR is preferably conducted in solid-phase. Capturing of mRNA and RT-PCR can be conducted in the same plates. The cDNA synthesized from the mRNA captured on the microplates can be used more than once. Further, in combination with the microplates, a filter plate is used for the preparation of cell lysates wherein target cells are placed on the filter plate, and a lysis buffer is passed through the cell layer on the filter to transfer cell lysate directly to the microplate via well-to-well communication.
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Access the complete Patent AT-E401415-T1: [Translated] DIRECT RT-PCR ON MICROTITER PLATES WITH IMMOBILIZED OLIGONUCLEOTIDE on the official website. |
https://pubchem.ncbi.nlm.nih.gov/patent/AT-E401415-T1 |
Tags
- patent
- chemistry
- research
- innovation
- pubchem