Ozone reacts with carbon black to produce a fulvic acid-like substance and increase an inflammatory effect

Figure 2. Cell non-heme iron concentrations following 4 (A) and 24 (B) hr exposures of BEAS-2B cells to media, carbon black treated with air (CB-Air), carbon black treated with ozone (CB-O3), and Suwanee River fulvic acid (SRFA). Incubations were without and with 200 μM FAC. Exposures to CB-Air, CB-O3, and SRFA did not impact cell non-heme iron relative to media at either time point. Exposure to FAC increased cell non-heme iron but co-exposure with CB-O3 was associated with significantly increased non-heme iron concentrations at both 4 (A) and 24 (B) hr. Exposures of BEAS-2B cells to FAC increased cell ferritin levels after 24 hr relative to media alone (C). However, co-exposure of CB-O3, and SRFA with FAC was associated with significantly elevated cell ferritin concentrations (C). Two-way ANOVA indicated that both addition of FAC and exposure group significantly impacted cell non-heme iron.

Figure 3. Release of IL-8 and IL-6 after 24 hr exposure of BEAS-2B cells to media, carbon black treated with air (CB-Air), carbon black treated with ozone (CB-O3), and Suwanee River fulvic acid (SRFA). Exposure to CB-O3 and SRFA significantly increased the release of IL-8 and IL-6. FAC treatment significantly diminished this response.

This dataset is associated with the following publication:
Ghio, A., D.H. Gonzalez, S.E.P. Paulson, J. Soukup, L. Dailey, M. Madden, B. Mahler, S.A. Elmore, M.C. Schladweiler, and U. Kodavanti. Ozone reacts with carbon black to produce a fulvic acid-like substance and increase an inflammatory effect. TOXICOLOGIC PATHOLOGY. Society of Toxicology, RESTON, VA, 48(7): 887-898, (2020).