Oyster Larval Survival Counts from Probiotic OY15 Experiments

Description

Environmentally-friendly methods for controlling microbial pathogenesis in aquaculture with probiotic bacteria are becoming increasingly preferred over use of chemical means, such as disinfectants or antibiotics. Recent research at the Milford Laboratory has shown that naturally-occurring bacteria isolated from the digestive glands of adult oysters, Crassostrea virginica, may be used as potential probiotic candidates in oyster larviculture. Probiotic candidate selection was based upon the Kirby Bauer Disc Diffusion Method, in which zones of inhibition suggested competitive exclusion of a known shellfish larvae pathogen (B183). In challenge studies, survival of 2-day old oyster larvae supplemented with probiotic candidate OY15 (Vibrio sp.) was similar (p<0.3883) to that of control larvae (no added bacteria), indicating no harmful effects. Further, addition of OY15 to oyster larvae challenged with B183 significantly improved survival (p<0.0141) compared to the pathogen alone. An in vitro study to determine the effects of OY15 or the pathogen B183 upon oyster hemocytes and their immune functions demonstrated immuno-stimulation of oyster hemocytes by OY15 and immune-suppression by B183, suggesting an immuno-modulation mechanism for OY15.

Resources

Name Format Description Link
0 View the complete metadata record on InPort for more information about this dataset. https://www.fisheries.noaa.gov/inport/item/26979
0 ISOLATION AND EVALUATION OF NEW PROBIOTIC BACTERIA FOR USE IN SHELLFISH HATCHERIES: I. ISOLATION AND SCREENING FOR BIOACTIVITY. Journal of Shellfish Research, Vol. 30, No. 3, 609–615, 2011 http://www.bioone.org/doi/full/10.2983/035.030.0303
0 ISOLATION AND EVALUATION OF NEW PROBIOTIC BACTERIA FOR USE IN SHELLFISH HATCHERIES: II. EFFECTS OF A VIBRIO SP. PROBIOTIC CANDIDATE UPON SURVIVAL OF OYSTER LARVAE (CRASSOSTREA VIRGINICA) IN PILOT-SCALE TRIALS. Journal of Shellfish Research, Vol. 30, No. 3, 617–625, 2011. http://www.bioone.org/doi/full/10.2983/035.030.0304
33 NOAA Data Management Plan for this record on InPort. https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/nefsc/dmp/pdf/26979.pdf
0 Global Change Master Directory (GCMD). 2024. GCMD Keywords, Version 19. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 View the complete metadata record on InPort for more information about this dataset. https://www.fisheries.noaa.gov/inport/item/26979
0 ISOLATION AND EVALUATION OF NEW PROBIOTIC BACTERIA FOR USE IN SHELLFISH HATCHERIES: I. ISOLATION AND SCREENING FOR BIOACTIVITY. Journal of Shellfish Research, Vol. 30, No. 3, 609–615, 2011 http://www.bioone.org/doi/full/10.2983/035.030.0303
0 ISOLATION AND EVALUATION OF NEW PROBIOTIC BACTERIA FOR USE IN SHELLFISH HATCHERIES: II. EFFECTS OF A VIBRIO SP. PROBIOTIC CANDIDATE UPON SURVIVAL OF OYSTER LARVAE (CRASSOSTREA VIRGINICA) IN PILOT-SCALE TRIALS. Journal of Shellfish Research, Vol. 30, No. 3, 617–625, 2011. http://www.bioone.org/doi/full/10.2983/035.030.0304
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 22. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
33 NOAA Data Management Plan for this record on InPort. https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/nefsc/dmp/pdf/26979.pdf

Tags

  • shellfish larvae
  • vibrio
  • disease prevention
  • doc/noaa/nmfs/nefsc
  • northeastern united states
  • noaa
  • northeast fisheries science center
  • oyster larviculture
  • u.s. department of commerce
  • national marine fisheries service
  • aquaculture sustainability branch (asb)
  • probiotic bacteria
  • crassostrea virginica
  • shellfish hatcheries
  • spring
  • larvae survival

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