Biphasic release propensity of mucin granules is supervised by tetraspanin-8 by sequestering syntaxin-2

Description

Agonist-mediated stimulated pathway of mucin and insulin release are biphasic in which rapid fusion of pre-docked granules is followed by slow docking and fusion of granules from the reserve pool. Here, based on a cell-culture system, we show that plasma membrane-located tetraspanin-8 sequesters syntaxin-2 to control mucin release. Tetraspanin-8 affects fusion of granules during the second phase of stimulated mucin release. The tetraspanin-8/syntaxin-2 complex does not contain VAMP-8, which functions with syntaxin-2 to mediate granule fusion. We suggest that by sequestering syntaxin-2, tetraspanin-8 prevents docking of granules from the reserve pool. In the absence of tetraspanin-8, more syntaxin-2 is available for docking and fusion of granules and thus doubles the quantities of mucins secreted. This principle also applies to insulin release and we suggest a cell type specific Tetraspanin/Syntaxin combination is a general mechanism regulating the fusion of dense core granules.

Resources

Name Format Description Link
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-7680682

Tags

  • airways
  • tspan-8
  • tetraspanin
  • syntaxin
  • mucin
  • lung
  • muc5-ac
  • snare
  • secretion

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