3D reconstruction of an NIH/3T3 mouse fibroblast cell imaged by the SXT-100.

This dataset (*.mrc file) is a reconstructed 3D volume obtained by soft X-ray tomography on NIH/3T3 cells. The cells were grown on a 200 mesh 3.05 mm EM finder grid with a Quantifoil Holey Carbon support. The sample was vitrified by plunge-freezing in liquid ethane. The tomogram was collected with a pixel size of 28.85 nm over the tilt range from -53 to 53.5 degrees with 1.5 deg step size and 116 s exposure per tilt.