Minimal dataset to test multiplexed DNA imaging (Hi-M) software pipelines

This is a dataset of nuclei (DAPI), and 3 multiplexed DNA imaging cycles to test and validate processing software packages, such as pyHiM (https://github.com/marcnol/pyHiM). This dataset was acquired in a nc14 Drosophila embryo. File contents: scan_001_RT27_001_ROI_converted_decon_ch00.tif     barcode 27, fiducial     scan_001_RT27_001_ROI_converted_decon_ch01.tif     barcode 27 scan_001_RT29_001_ROI_converted_decon_ch00.tif     barcode 29, fiducial           scan_001_RT29_001_ROI_converted_decon_ch01.tif     barcode 29      scan_001_RT37_001_ROI_converted_decon_ch00.tif     barcode 37, fiducial              scan_001_RT37_001_ROI_converted_decon_ch01.tif     barcode 37        scan_006_DAPI_001_ROI_converted_decon_ch00.tif     DAPI      scan_006_DAPI_001_ROI_converted_decon_ch01.tif     DAPI, fiducial         scan_006_DAPI_001_ROI_converted_decon_ch02.tif     RNA   To test this dataset please refer to pyHiM documentation page.