Probiotic Bacillus subtilis Protects against a-Synuclein Aggregation in C. elegans (fluorescence microscopy data)

Description

This project has been submitted by the Maria Doitsidou Lab. Project contents: This project contains datasets of z-stack images of C. elegans strains used to study how the gut microbiome affects Parkinson’s disease. Each strain contains a chromosomal insertion containing YFP fused to α-synuclein (pkIs2386[Punc-54::α-synuclein::YFP + unc-119(+)]). The following C. elegans strains were used and/or created for this project: NL5901 pkIs2386[Punc-54::α-synuclein::YFP + unc-119(+)] MDH586 daf-2(e1370) III; pkIs2386 MDH585 daf-16(mu86) I; pkIs2386 MDH587 hsf-1(sy441) I; pkIs2386 MDH657 daf-2(e1370) III; daf-16(mu86) I; pkIs2386 MDH614 daf-2(gk390525) III; pkIs2386 MDH611 eat-2(ad465) II; pkIs2386 MDH711 lagr-1(gk331) I, pkIs2386 MDH725 sptl-3(ok1927) II; pkIs2386 MDH724 asm-3(ok1744) IV; pkIs2386. High magnification (40x objective) z stack images of the head region were obtained by using a Zeiss Axio imager 2 microscope. Aim: Study how a probiotic B. subtilis strain affects alpha-synuclein protein aggregation. Main results: The authors showed that the probiotic B. subtilis strain PXN21 inhibits and clears a-synuclein aggregation in a C. elegans model. The bacterium acts via metabolites and biofilm formation to activate protective pathways in the host, including DAF-16/FOXO and sphingolipid metabolism. Contributors: Maria Eugenia Goya, Feng Xue, Cristina Sampedro-Torres-Quevedo, Sofia Arnaouteli, Lourdes Riquelme-Dominguez, Andres Romanowski, Jack Brydon, Kathryn L. Ball, Nicola R. Stanley-Wall and Maria Doitsidou These datasets were used in the following publication: Probiotic Bacillus subtilis Protects against a-Synuclein Aggregation in C. elegans Maria Eugenia Goya, Feng Xue, Cristina Sampedro-Torres-Quevedo, Sofia Arnaouteli, Lourdes Riquelme-Dominguez, Andres Romanowski, Jack Brydon, Kathryn L. Ball, Nicola R. Stanley-Wall and Maria Doitsidou Cell Reports January 14, 2020 30 367-380; first published January 14, 2020 https://doi.org/10.1016/j.celrep.2019.12.078

Resources

Name Format Description Link
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-5546323
0 http://data.europa.eu/88u/dataset/oai-zenodo-org-5546323

Tags

  • daf-16-foxo
  • sphingolipid-metabolism
  • parkinson's-disease
  • b.-subtilis
  • α-synuclein
  • probiotics
  • microbiota
  • dietary-restriction
  • biofilm
  • c.-elegans

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