Phenotype profile of monocytes differentiated in the presence of semen Fucosylated Clusterin

Having shown that fucosylated clusterin effectively interacts with macrophages via DC-SIGN, we then analyzed its influence on the phenotype of macrophages. In these experiments, monocytes were cultured for 5 days with M-CSF, in the absence or presence of semen fucosylated clusterin. Then, the cells were cultured for additional 24 hours with or without LPS and the phenotype were analyzed. Fucosylated clusterin-treated macrophages failed to upregulate the expression of HLA-DR and CD86 induced by LPS but not the expression of CD40, CD80 and PDL-1. These results are represented on Figure 6 from the publication: Aberrant fucosylation enables breast cancer clusterin to interact with dendritic cell-specific ICAM-grabbing non-integrin (DC-SIGN).