NCCOS Assessment: Monitoring Increases of Amoxicillin Resistance in Coral Mucus Bacteria under Laboratory Conditions at Charleston, SC, 2022-02-01 to 2024-09-30 (NCEI Accession 0306277)

Description

The data set includes data for the development and testing for experiments that measure increases in amoxicillin resistance of bacterial mucus cultures. MIC (minimum inhibitory concentration) data – These experiments were used to establish the lowest amoxicillin concentration at which no bacteria would grow in liquid cultures. Optical density readings from a spectrophotometer were used to monitor the growth of the coral mucus cultures with higher readings indicative of more growth. The table includes data from two separate replicate experiments using four different amoxicillin concentrations. Once established, concentrations at or below the MIC level were used to regulate the amount of selection pressure applied to cultures. Standard Growth Curve Data – Standard growth curves were created for coral mucus cultures that were grown at 0 and 40 mg/L amoxicillin over a seven-hour period. The spreadsheet contains, hourly optical density timepoints taken over the seven-hour period. At each timepoint, culture dilutions were also made and plated to agar plates and grown overnight. The optical density readings and the resulting CFU plate counts were paired together to generate the CFU/mL concentration column. This column was then used to create regression line equations for both the 0 and 40 mg/L cultures and that could be used in the future to approximate the cell concentration of a culture from its optical density reading. This was important for culture passaging during multi-day experiments. Live Coral Data – Data that was used to assess how treating live coral in artificial seawater with an amoxicillin-loaded delivery paste influences antibiotic resistance over a 28-day period. Data in table are the percent resistant colonies (plated to 400 mg/L) out of the total number of colonies plated from the beginning (day 1), middle (day 14) and end (day 28) of the study. Doubling Time Data – The doubling time of bacteria (the time it takes for a bacterium to divide) was used to access antibiotic resistance levels. Data in the table are optical density readings taken every hour from each treatment over a seven-hour period from the beginning (day 1) and ending (day 28) of the experiment. Liquid Inhibition Data – Growing coral mucus bacteria with and without amoxicillin, then plating on different levels of selection to gauge levels of amoxicillin resistance. Data in the table are CFU plate counts from a culture grown for 28-days in 0 mg/L amoxicillin and a culture grown for 28-days in 40 mg/L amoxicillin. At the 1, 14 and 28-day timepoints, the two cultures were plated to selective plates containing 0, 100 and 400 mg/L amoxicillin. The average number of CFU that were present for each culture at each plate concentration are presented in the table. Amoxicillin Leaching Water Quality Data – Data in the table are the pH, temperature and salinity data for each treatment replicate for the 7-day amoxicillin leaching experiment. Live Coral Challenge Water Quality Data - Data in the table are the pH, temperature and salinity data for each treatment replicate for the 28-day live coral challenge experiment.

Resources

Name Format Description Link
0 Institution web page https://coralreef.noaa.gov
0 Institution web page https://coralreef.noaa.gov
0 Navigate directly to the URL for a descriptive web page with download links. https://www.ncei.noaa.gov/archive/accession/0306277
0 Navigate directly to the URL for a descriptive web page. https://www.ncei.noaa.gov/archive/accession/oas/306277
0 Navigate directly to the URL for a descriptive web page. https://www.ncei.noaa.gov/archive/accession/download/306277
0 Users may contact NCEI for more information and to request access to the archived data. User requests must be approved by NCEI and are subject to order fulfillment constraints. https://www.ncei.noaa.gov/contact
0 Information for contacts at NCEI. https://www.ncei.noaa.gov/contact
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://www.css-inc.com/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://www.css-inc.com/
0 Institution web page https://www.css-inc.com/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coralreef.noaa.gov
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coralreef.noaa.gov
0 Information for contacts at NCEI. https://www.ncei.noaa.gov/contact
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 NOAA Technical Memorandum https://doi.org/10.25923/temw-nd20
0 Navigate directly to the URL for a descriptive web page with download links. https://doi.org/10.25921/3sfx-h257
0 Navigate directly to the URL for a descriptive web page with download links. https://www.ncei.noaa.gov/archive/accession/oas/306277
0 Navigate directly to the URL for data access and direct download. https://www.ncei.noaa.gov/archive/accession/download/306277
0 Information for contacts at NCEI. https://www.ncei.noaa.gov/contact
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coralreef.noaa.gov
0 Institution web page https://coralreef.noaa.gov
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://www.css-inc.com/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://www.css-inc.com/
0 Institution web page https://www.css-inc.com/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coralreef.noaa.gov
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coralreef.noaa.gov
0 Information for contacts at NCEI. https://www.ncei.noaa.gov/contact
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 NOAA Technical Memorandum https://doi.org/10.25923/temw-nd20
0 These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0235/0306277/
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Institution web page https://coastalscience.noaa.gov/
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Institution web page https://coralreef.noaa.gov
0 Institution web page https://www.css-inc.com/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://www.css-inc.com/
0 Navigate directly to the URL for a descriptive web page with download links. https://doi.org/10.25921/3sfx-h257
0 Navigate directly to the URL for a descriptive web page with download links. https://www.ncei.noaa.gov/archive/accession/oas/306277
0 Navigate directly to the URL for data access and direct download. https://www.ncei.noaa.gov/archive/accession/download/306277
0 These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0235/0306277/
0 Information for contacts at NCEI. https://www.ncei.noaa.gov/contact
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coralreef.noaa.gov
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coralreef.noaa.gov
0 Institution web page https://coastalscience.noaa.gov/
0 Institution web page https://coralreef.noaa.gov
0 Information for contacts at NCEI. https://www.ncei.noaa.gov/contact
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 NOAA Technical Memorandum https://doi.org/10.25923/temw-nd20
0 Institution web page https://www.css-inc.com/
0 Institution web page https://coastalscience.noaa.gov/
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords

Tags

  • coastal change
  • agar plates
  • florida reef tract
  • united states of america
  • orion star a211 benchtop meter (thermo fisher)
  • live coral data
  • bacteria
  • corals
  • orbicella faveolata
  • biosphere
  • standard curve data
  • esa listed species
  • live coral water quality
  • monroe county
  • waterbodies
  • noaa coral reef conservation program – project 31373 – “effects analysis for coral disease tre
  • numeric data sets
  • oceanography
  • biology
  • minimum inhibitory concentration
  • liquid inhibition data
  • scleractinia (stony corals)
  • stony coral tissue loss disease
  • atlantic ocean
  • earth science
  • effects analysis for coral disease treatments
  • amoxicillin
  • florida keys (24n081w0007)
  • leach water quality
  • coral diseases
  • 31373
  • microbiota
  • florida reef tract (26n080w0007)
  • florida coast
  • florida
  • thermo scientific)
  • u.s. states and territories
  • ocean basin
  • restoration
  • doubling time data
  • laboratory experiment
  • spectrophotometer (spectronic 20d+
  • 95m7bu
  • zoology
  • coral condition
  • north atlantic ocean
  • antibiotic resistance
  • vulnerability and risk assessment
  • country/territory

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