Multiplatform metabolomic interlaboratory study of a whole human stool candidate reference material from omnivore and vegan donors
Description
The repository contains the analysis and results collected during a metabolomics interlaboratory study. The study involved the experimental analysis of human stool from vegan and omnivore donors preserved in lyophilized format and aqueous phase, all stored at -80 C. The interlaboratory study (ILS) involved 18 participant institutions, and they were given the flexibility to use their preferred sample preparation method and analytical platform to analyze the stool samples. The analytical platforms used in the study were liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) to analyze the stool samples.Each participant submitted two lists of metabolites - one containing the Top 20 most abundant metabolites, and the other containing all annotated metabolites. The collected data was then analyzed to assess the suitability of a suite of candidate fecal reference material, ultimately aiming to enhance confidence in the measurements of gut metabolomics.
Resources
| Name |
Format |
Description |
Link |
|
47 |
|
https://data.nist.gov/od/ds/ark:/88434/mds2-3104/Data.zip.sha256 |
|
57 |
ILS%20Participants.zip |
https://data.nist.gov/od/ds/mds2-3104/ILS%20Participants.zip |
|
57 |
Data.zip |
https://data.nist.gov/od/ds/ark:/88434/mds2-3104/Data.zip |
Tags
- multiplatform-analysis-and-metabolomics
- feces
- human-stool
- gut-metabolomics
- standards