Multi-omic investigations of mouse liver subjected to simulated spaceflight freezing and storage protocols
Description
This study compares standard laboratory protocols for tissue freezing and storage with a simulation of the delayed processing of liver specimens and long-term storage protocols used during the Rodent Research-1 (RR-1) payload. Liver samples from twelve-week old female C57BL/6J mice (snap frozen vs. 25 min. slow-frozen and stored for 3 days vs. 1 year) received from the RR-1 project were processed for extraction of RNA DNA and protein. RNA-seq whole genome bisulfite sequencing and mass spectrometry proteomics were performed. These multi-omics datasets will help distinguish the effects of freezing and storage time that could confound the interpretation of the Rodent Research-1 datasets.
Resources
| Name |
Format |
Description |
Link |
|
21 |
GeneLab Study Page |
https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-49 |
|
21 |
GeneLab Study Page |
https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-49 |
Tags
- sample-collection
- protein-extraction
- sample-storage-time
- nucleic-acid-extraction
- data-transformation
- animal-and-tissue-sampling
- tmt-isobaric-labeling
- extraction
- labeling
- sample-preservation-method
- library-construction
- nucleic-acid-sequencing
- genelab-rnaseq-data-processing-protocol
- mass-spectrometry
- sequencing-library-construction
- freezing-profile