Molecular and morphological data to resolve species boundaries in the critically imperiled freshwater mussel species, Fusconaia mitchelli

We generated multilocus DNA sequence data and traditional morphometric measurements to evaluate species boundaries in Fusconaia mitchelli. We sequenced three loci: the protein-coding mitochondrial DNA genes cytochrome oxidase subunit 1 and NADH dehydrogenase 1, and the nuclear ribosomal internal transcribed spacer 1. We also took three measurements, maximum length, maximum width, and maximum height, of mussel shells for morphometric analysis.