Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection
Description
To reveal outcomes of microgravity on molecular processes within the cellular environment we have employed a mass-spectrometry based proteomics approach. Proteomics analysis based on mass spectrometry allows for the relative quantitation of a large number of proteins concurrently, and in a relatively unbiased manner. Mass spectrometry based proteomics can be rendered even more informative by addition of a labeling component to understand the dynamics of the changing protein content. In this study, we utilized a combination of proteomics techniques, namely label-free quantification and dynamic stable-isotope labeling by amino acids in cell culture (Dynamic SILAC) to characterize the microgravity stress response in primary cardiomyocytes.
Resources
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https://lsda.jsc.nasa.gov%2Fscripts%2Fexperiment%2Fexper.aspx%3Fexp_index%3D2093 |
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5 |
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https://osdr.nasa.gov%2Fbio%2Frepo%2Fdata%2Fstudies%2FOSD-122 |
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5 |
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https://lsda.jsc.nasa.gov/scripts/experiment/exper.aspx?exp_index=2093 |
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5 |
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https://osdr.nasa.gov/bio/repo/data/studies/OSD-122 |