Microbial Observatory (ISS-MO): Study of BSL-2 bacterial isolates from the International Space Station
Description
In an on-going Microbial Observatory experimental investigation on the International Space Station (ISS) multiple bacterial isolates of Biosafety Level 2 (BSL-2) were isolated and identified. The antibiotic susceptibility pattern was tested in these BSL-2 isolates for the following antibiotics: cefazolin ciprofloxacin cefoxitin erythromycin gentamycin oxacillin penicillin rifampin tobramycin and many of the BSL-2 isolates showed multiple drug resistance. Among these isolates 21 strains were chosen for whole genome sequencing (WGS) for a possible lead to develop appropriate countermeasures. In addition the genomic data would enable to determine the influence of microgravity on the pathogenicity and virulence in the BSL-2 microorganisms.
Resources
| Name |
Format |
Description |
Link |
|
21 |
GeneLab Study Page |
https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-67 |
Tags
- sample-collection
- nucleic-acid-extraction
- nucleic-acid-extraction-was-done-usinq-qiagen-dna-extraction-kit-as-per-manufacturer-instructio
- microgravity
- illumina-nextseq
- truseq-dna-pcr-free-was-used-as-per-manufacturer-instruction
- library-construction
- sequence-assembly
- nucleic-acid-sequencing
- sequence-assembly-using-a5-genome-assembler
- sample-collection-was-done-using-sterile-moisten-wipes