MAM Consortium Interlaboratory Study Raw Data

Description

These LC-MS and LC-MS/MS raw data were collected for purposes of an interlaboratory study evaluating the multi-attribute method (MAM). Tryptic digests of native NISTmAb (the "Reference" and the "Unknown" samples), degraded NISTmAb (the "pH Stress" sample) and NISTmAb spiked with 15 heavy-labeled synthetic peptides (the "Spike" sample) were sent to each participating laboratory. One injection of each digest was acquired in MS-only mode, while a second injection was acquired in MS/MS mode. Three injections of a mixture of 15 heavy-labeled synthetic peptides ("Calibration" sample) were also analyzed as a means of evaluating instrument performance. Although all data were collected using the same C18 column and LC method, the instrumentation used by each laboratory differed. Additional details regarding the samples, their preparation, the LC method used, and an evaluation of the results pertaining to the new peak detection aspect of MAM can be found in "New Peak Detection Performance Metrics from the MAM Consortium Interlaboratory Study" (https://pubs.acs.org/doi/10.1021/jasms.0c00415). Evaluation of the results pertaining to the attribute analytics aspect of MAM can be found in "Interlaboratory Attribute Analytics Metrics from the MAM Consortium Round Robin Study" (link to be provided). Raw data that was optionally submitted by sixteen participating laboratories are provided here. To preserve the integrity of the data, the files are provided in their original vendor format.Please note that Linux and Mac users may require the use of 7Zip (https://www.7-zip.org/) to extract zipped folders, rather than the extraction tool provided by their operating system.For any difficulty with downloading or extracting data files, please e-mail the contact listed above.

Resources

Name Format Description Link
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_09.zip.sha256
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_05.zip.sha256
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_07.zip.sha256
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_01.zip.sha256
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_03.zip.sha256
57 MAM_Data_04.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_04.zip
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_04.zip.sha256
57 MAM_Data_05.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_05.zip
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_10.zip.sha256
57 MAM_Data_12.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_12.zip
57 MAM_Data_14.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_14.zip
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_14.zip.sha256
57 MAM_Data_15.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_15.zip
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_15.zip.sha256
57 MAM_Data_16.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_16.zip
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_16.zip.sha256
57 MAM_Data_02.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_02.zip
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_02.zip.sha256
57 MAM_Data_08.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_08.zip
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_11.zip.sha256
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_13.zip.sha256
0 Amino acid sequences relevant to raw data https://data.nist.gov/od/ds/mds2-2497/FASTA%20Sequence.fasta
47 README_2022-07-12.txt https://data.nist.gov/od/ds/mds2-2497/README_2022-07-12.txt
57 MAM_Data_10.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_10.zip
57 MAM_Data_09.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_09.zip
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_12.zip.sha256
57 MAM_Data_01.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_01.zip
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_08.zip.sha256
57 MAM_Data_11.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_11.zip
57 MAM_Data_13.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_13.zip
57 MAM_Data_07.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_07.zip
57 MAM_Data_06.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_06.zip
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_06.zip.sha256
57 MAM_Data_03.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2497/MAM_Data_03.zip

Tags

  • round-robin
  • nistmab
  • npd
  • attribute-analytics
  • purity-testing
  • new-peak-detection
  • targeted-analytics
  • interlaboratory-study
  • multi-attribute-method
  • mass-spectrometry
  • mam-consortium

Topics

Categories