Lipophilic Toxins in WA - Clear and present danger: monitoring and management of lipophilic shellfish toxins in Washington State

Description

Lipophilic shellfish toxins comprise an extensive suite of compounds including those associated with the human syndromes known as diarrhetic shellfish poisoning (DSP) and azaspiracid shellfish poisoning (AZP). As a result of recent bloom events and subsequent human intoxications in Washington State (USA) due to DSP, there is a critical and urgent need for State public health officials to be able to monitor and accurately quantify harmful algal bloom (HAB) species associated with DSP and azaspiracid shellfish poisoning (AZP) and their toxins. There is now evidence that lipophilic toxins associated with DSP and AZP are present in water and/or shellfish, including oysters and mussels from Puget Sound and razor clams from the WA coast.Tight partnership with WDOH, the SoundToxins program, Olympic Region Harmful Algal Blooms (ORHAB) partnership, and Puget Sound shellfish growers (including the Jamestown S’Klallam tribe and other tribal representatives) will facilitate the study of the seasonal variability of lipophilic toxins and toxin-producing species at 10 geographically-distinct sites within Washington State waters where seawater or shellfish have recently been contaminated with these toxins. Stakeholder support throughout the project will ensure the transition of this project to the State at the end of 3 years as we have successfully demonstrated with ORHAB. Implementing routine lipophilic biotoxin monitoring will be a critical first step towards ensuring public safety while also enabling Washington State shellfish growers to sell their product to the European Union once trade is re-established. Contains information regarding toxin producing species and toxin measurements in shellfish and seawater samples. Also includes associated environmental data.

Resources

Name Format Description Link
0 Site names, coordinates, dates, and times that shellfish samples were collected for lipophilic marine toxin analyses. The results liquid chromatography with tandem mass spectroscopy (LC-MS/MS) analyses for the toxins (okadaic acid, dinophysis toxin 1, dinophysis toxin 2, azaspiracids 1, azaspiracid 2, azaspiracid 3, as well as total OA equivalents) are included. Additionally, the results of a screening assay for lipophilic toxins (PP2A) that detects okadaic acid and the dinophysis toxins are included. https://www.webapps.nwfsc.noaa.gov/apex/parr/aza_project_shellfish_data_for_lipophilic_toxins_in_washington_state_2016/data/page/
33 NOAA Data Management Plan for this record on InPort. https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/nwfsc/dmp/pdf/30880.pdf
0 View the complete metadata record on InPort for more information about this dataset. https://www.fisheries.noaa.gov/inport/item/30880
0 Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample. https://www.webapps.nwfsc.noaa.gov/apex/parr/aza_project_seawater_date_for_lipophilic_toxins_in_washington_state_2016/data/page/
0 Site names, coordinates, dates, and times that shellfish samples were collected for lipophilic marine toxin analyses. The results liquid chromatography with tandem mass spectroscopy (LC-MS/MS) analyses for the toxins (okadaic acid, dinophysis toxin 1, dinophysis toxin 2, azaspiracids 1, azaspiracid 2, azaspiracid 3, as well as total OA equivalents) are included. Additionally, the results of a screening assay for lipophilic toxins (PP2A) that detects okadaic acid and the dinophysis toxins are included. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/aza_project_shellfish_data_for_lipophilic_toxins_in_washington_state_2016
0 NWFSC Dataset Information page. Contains information regarding toxin producing species and toxin measurements in shellfish and seawater samples. Also includes associated environmental data. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/datasets/dataset/104746
0 Global Change Master Directory (GCMD). 2024. GCMD Keywords, Version 19. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/aza_project_seawater_date_for_lipophilic_toxins_in_washington_state_2016
0 Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample. https://www.webapps.nwfsc.noaa.gov/apex/parr/aza_project_seawater_date_for_lipophilic_toxins_in_washington_state_2016/data/page/
0 Site names, coordinates, dates, and times that shellfish samples were collected for lipophilic marine toxin analyses. The results liquid chromatography with tandem mass spectroscopy (LC-MS/MS) analyses for the toxins (okadaic acid, dinophysis toxin 1, dinophysis toxin 2, azaspiracids 1, azaspiracid 2, azaspiracid 3, as well as total OA equivalents) are included. Additionally, the results of a screening assay for lipophilic toxins (PP2A) that detects okadaic acid and the dinophysis toxins are included. https://www.webapps.nwfsc.noaa.gov/apex/parr/aza_project_shellfish_data_for_lipophilic_toxins_in_washington_state_2016/data/page/
0 View the complete metadata record on InPort for more information about this dataset. https://www.fisheries.noaa.gov/inport/item/30880
0 NWFSC Dataset Information page. Contains information regarding toxin producing species and toxin measurements in shellfish and seawater samples. Also includes associated environmental data. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/datasets/dataset/104746
0 Global Change Master Directory (GCMD). 2024. GCMD Keywords, Version 19. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
33 NOAA Data Management Plan for this record on InPort. https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/nwfsc/dmp/pdf/30880.pdf
0 Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/aza_project_seawater_date_for_lipophilic_toxins_in_washington_state_2016
0 Site names, coordinates, dates, and times that shellfish samples were collected for lipophilic marine toxin analyses. The results liquid chromatography with tandem mass spectroscopy (LC-MS/MS) analyses for the toxins (okadaic acid, dinophysis toxin 1, dinophysis toxin 2, azaspiracids 1, azaspiracid 2, azaspiracid 3, as well as total OA equivalents) are included. Additionally, the results of a screening assay for lipophilic toxins (PP2A) that detects okadaic acid and the dinophysis toxins are included. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/aza_project_shellfish_data_for_lipophilic_toxins_in_washington_state_2016
0 Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/aza_project_seawater_date_for_lipophilic_toxins_in_washington_state_2016
0 Site names, coordinates, dates and times that water samples were collected for analysis of three Azadinium species as well as the family Amphidomataceae. Cell enumeration results are included for the three Azadinium species (A. spinosum, A. poporum and A. obesum) as determinted by real-time quantitative PCR using TaqMan probe assays. Additionally, the results of a SYBR green based quantitative PCR assay are included that indicate whether or not members of the family Amphidomataceae are present in a water sample. https://www.webapps.nwfsc.noaa.gov/apex/parr/aza_project_seawater_date_for_lipophilic_toxins_in_washington_state_2016/data/page/
0 Site names, coordinates, dates, and times that shellfish samples were collected for lipophilic marine toxin analyses. The results liquid chromatography with tandem mass spectroscopy (LC-MS/MS) analyses for the toxins (okadaic acid, dinophysis toxin 1, dinophysis toxin 2, azaspiracids 1, azaspiracid 2, azaspiracid 3, as well as total OA equivalents) are included. Additionally, the results of a screening assay for lipophilic toxins (PP2A) that detects okadaic acid and the dinophysis toxins are included. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/aza_project_shellfish_data_for_lipophilic_toxins_in_washington_state_2016
0 Site names, coordinates, dates, and times that shellfish samples were collected for lipophilic marine toxin analyses. The results liquid chromatography with tandem mass spectroscopy (LC-MS/MS) analyses for the toxins (okadaic acid, dinophysis toxin 1, dinophysis toxin 2, azaspiracids 1, azaspiracid 2, azaspiracid 3, as well as total OA equivalents) are included. Additionally, the results of a screening assay for lipophilic toxins (PP2A) that detects okadaic acid and the dinophysis toxins are included. https://www.webapps.nwfsc.noaa.gov/apex/parr/aza_project_shellfish_data_for_lipophilic_toxins_in_washington_state_2016/data/page/
0 View the complete metadata record on InPort for more information about this dataset. https://www.fisheries.noaa.gov/inport/item/30880
0 NWFSC Dataset Information page. Contains information regarding toxin producing species and toxin measurements in shellfish and seawater samples. Also includes associated environmental data. https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/datasets/dataset/104746
0 Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 22. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
33 NOAA Data Management Plan for this record on InPort. https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/nwfsc/dmp/pdf/30880.pdf

Tags

  • animal and plant collection device
  • sonde and probe - conductivity
  • fishery-dependent
  • azaspiracids
  • and depth
  • northwest fisheries science center
  • tracked asset
  • azadinium
  • chemistry
  • in-situ instrument - in-situ instrument (not specified)
  • nwfsc montlake laboratory
  • doc/noaa/nmfs/nwfsc
  • diarrhetic shellfish toxins
  • national marine fisheries service
  • noaa. u.s. department of commerce
  • animal and plant collection device - plankton collection device - plankton net
  • dinophysis
  • efs (environmental and fisheries sciences) division
  • various locations in wa
  • temperature
  • toxic effects
  • water sampler bottle - surface sample bottle
  • biotoxins
  • marine toxin

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