Image data of growth of Valencia sweet orange nonembryogenic cells on points from a 5-factor response surface design to determine the effects of mineral nutrition on growth

The data are images of tissue cultures of Valencia sweet orange nonembryogenic callus cells taken in 2006 at the U.S. Horticultural Research Laboratory, Ft. Pierce, Florida, USA to photo document treatments from an experiment designed to determine the effects of mineral nutrition on callus growth using a 5-factor response surface methodology (RSM) design. The design matrix is presented in the spreadsheet file 5 factor RSM Design_11-28-2005.xlsx. The design included 46 runs divided into 3 blocks. Six culture dishes were used to estimate the response for each run. A culture dish representative of the run was photographed. Each image is named to match the run of the design. For example, the jpg image labeled Run 04_Blk1_5F RSM_NE_Valencia_11-28-2005.JPG is an image of the callus grown on Run #4 that was part of Block 1 of the 5-factor RSM design for the nonembryogenic Valencia sweet orange callus cells. This dataset includes 47 files – 46 image files and 1 Excel spreadsheet. Images were captured in JPEG (EXIF 2.2) format with a Nikon Coolpix 5400 digital camera equipped with a 1/1.8” (7.2 x 5.3 mm) CCD sensor at a resolution of 2592 x 1944 pixels. Each image is of a single culture plate with the top lid removed and photographed under cool white, fluorescent lighting.

The experimental setup for the 5-factor response surface design is described in - Niedz, R. P. and T. J. Evens (2007). "Regulating plant tissue growth by mineral nutrition." In Vitro Cellular & Developmental Biology - Plant 43(4): 370-381.