Spatiotemporally controlled generation of NTPs for single-molecule studies of NTP-driven processes

Description

Data supporting the findings of the paper titled "Spatiotemporally controlled generation of NTPs for single-molecule studies" in Nature Chemical Biology which describes a method for controlling NTP-driven reactions in single-molecule experiments via the local generation of NTPs (LAGOON) that increases the measurement throughput and enables single-turnover observations.  The dataset contains single-molecule FRET data capturing T4 helicase DNA unwinding, nucleosome remodeling by Chd1 and the invasion of a nucleosome by the T7 RNA polymerase. Additionally, the dataset contains acoustic force spectroscopy data on E. coli RNA polymerase elongation.

Resources

Name Format Description Link
0 http://data.europa.eu/88u/dataset/https-doi-org-10-17044-scilifelab-20107199

Tags

  • biochemistry-and-cell-biology-not-elsewhere-classified
  • dataset
  • enzymes

Topics

  • HEAL

Categories