High-volume, label-free imaging for quantifying single-cell dynamics in iPSC colonies

Description

This collection is comprised of multiple sets of experiments involving iPSCs with varying dosages of fluorescent light excitation. Samples were imaged every 2 minutes using phase-contrast microscopy for 20+ hours with or without fluorescence excitation. Each experiment was done on different days (i.e. exp0 vs exp1) but with triplicate wells (i.e. exp1-0, exp1-1, exp1-2). Additionally, data used to train our 2D U-Net and 3D U-Net models are included. Detailed information for each dataset can be found in Dataset_Information.xlsx.

Resources

Name Format Description Link
57 exp3-0.zip https://data.nist.gov/od/ds/mds2-2960/exp3-0.zip
57 exp2-4.zip https://data.nist.gov/od/ds/mds2-2960/exp2-4.zip
57 exp3-3.zip https://data.nist.gov/od/ds/mds2-2960/exp3-3.zip
57 exp3-4.zip https://data.nist.gov/od/ds/mds2-2960/exp3-4.zip
57 exp0.zip https://data.nist.gov/od/ds/mds2-2960/exp0.zip
57 exp6-0.zip https://data.nist.gov/od/ds/mds2-2960/exp6-0.zip
57 exp6-1.zip https://data.nist.gov/od/ds/mds2-2960/exp6-1.zip
57 exp4-5.zip https://data.nist.gov/od/ds/mds2-2960/exp4-5.zip
57 exp4-0.zip https://data.nist.gov/od/ds/mds2-2960/exp4-0.zip
57 exp4-4.zip https://data.nist.gov/od/ds/mds2-2960/exp4-4.zip
57 exp5-2.zip https://data.nist.gov/od/ds/mds2-2960/exp5-2.zip
57 exp5-4.zip https://data.nist.gov/od/ds/mds2-2960/exp5-4.zip
57 exp5-5.zip https://data.nist.gov/od/ds/mds2-2960/exp5-5.zip
57 training_low.zip https://data.nist.gov/od/ds/mds2-2960/training_low.zip
57 training_3DUNET.zip https://data.nist.gov/od/ds/mds2-2960/training_3DUNET.zip
57 exp2-1.zip https://data.nist.gov/od/ds/mds2-2960/exp2-1.zip
57 exp2-5.zip https://data.nist.gov/od/ds/mds2-2960/exp2-5.zip
57 exp1.zip https://data.nist.gov/od/ds/mds2-2960/exp1.zip
57 exp3-2.zip https://data.nist.gov/od/ds/mds2-2960/exp3-2.zip
57 training_medium.zip https://data.nist.gov/od/ds/mds2-2960/training_medium.zip
57 exp2-2.zip https://data.nist.gov/od/ds/mds2-2960/exp2-2.zip
57 training_high.zip https://data.nist.gov/od/ds/mds2-2960/training_high.zip
37 GitHub page with all scripts and models used for the image dataset. https://github.com/usnistgov/WSDOM
57 exp4-1.zip https://data.nist.gov/od/ds/mds2-2960/exp4-1.zip
57 exp5-0.zip https://data.nist.gov/od/ds/mds2-2960/exp5-0.zip
47 https://data.nist.gov/od/ds/ark:/88434/mds2-2960/exp7.zip.sha256
57 exp2-0.zip https://data.nist.gov/od/ds/mds2-2960/exp2-0.zip
57 exp2-3.zip https://data.nist.gov/od/ds/mds2-2960/exp2-3.zip
57 exp3-1.zip https://data.nist.gov/od/ds/mds2-2960/exp3-1.zip
57 exp4-2.zip https://data.nist.gov/od/ds/mds2-2960/exp4-2.zip
57 exp4-3.zip https://data.nist.gov/od/ds/mds2-2960/exp4-3.zip
57 exp5-1.zip https://data.nist.gov/od/ds/mds2-2960/exp5-1.zip
57 exp5-3.zip https://data.nist.gov/od/ds/mds2-2960/exp5-3.zip
57 exp3-5.zip https://data.nist.gov/od/ds/mds2-2960/exp3-5.zip
53 Dataset_Information.xlsx https://data.nist.gov/od/ds/mds2-2960/Dataset_Information.xlsx
57 exp7.zip https://data.nist.gov/od/ds/ark:/88434/mds2-2960/exp7.zip

Tags

  • genetic-engineering-regenerative-medicine
  • u-net
  • ai
  • pluripotent-cell-lines-quantitative-live-cell-imaging-fluorescence-microscopy-image-data-analys

Topics

Categories