HARV replication of the BRIC-23 Bacillus subtilis spaceflight

Description

Previous spaceflight experiments using Bacillus subtilis have reported altered transcriptome profiles during spaceflight compared to matching ground samples. This study tried to replicate those transcriptome changes using High Aspect Ratio Vessels (HARVs). B. subtilis spores were grown in HARVs using the same protocol and growth conditions as the BRIC-23 spaceflight mission. RNA was extracted from the HARV samples and sequenced for differential expression analysis. HARV differentially expressed genes were then compared to the genes identified as differentially expressed in the BRIC-23 mission to access the accuracy of HARVs as a model for spaceflight experiments.

Resources

Name Format Description Link
21 GeneLab Study Page https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-216
21 GeneLab Study Page https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-144
21 Search results for publications that cite this dataset by its DOI. https://scholar.google.com/scholar?q=10.5067%2FESDQS-L2W10
21 Information on PO.DAAC's Designated MEaSUREs Projects https://podaac.jpl.nasa.gov/measures
21 Project Description https://earthdata.nasa.gov/esds/competitive-programs/measures/esdr-ocean-surface-winds-1999-2022
21 README file https://archive.podaac.earthdata.nasa.gov/podaac-ops-cumulus-docs/measures_ocean_surface_wind_vectors/preview/L2/scatterometer_data/quikscat/README.txt
21 Generic Data Readers https://github.com/podaac/data-readers
21 QuikSCAT Mission Page at PO.DAAC http://podaac.jpl.nasa.gov/QuikSCAT
22 Thumbnail https://podaac.jpl.nasa.gov/Podaac/thumbnails/QUIKSCAT_ESDR_L2_WIND_STRESS_V1.0.jpg
21 README file https://archive.podaac.earthdata.nasa.gov/podaac-ops-cumulus-docs/measures_ocean_surface_wind_vectors/preview/L2/scatterometer_data/quikscat/README.txt
21 Data Use and Citation Policy https://podaac.jpl.nasa.gov/CitingPODAAC
21 HTTPS endpoint for data browse and download https://cmr.earthdata.nasa.gov/virtual-directory/collections/C2491137146-POCLOUD
21 Browse granule search results in Earthdata Search https://search.earthdata.nasa.gov/search/granules?p=C2491137146-POCLOUD
21 GeneLab Study Page https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-216

Tags

  • nucleic-acid-extraction
  • treatment-protocol
  • library-construction
  • nucleic-acid-sequencing
  • microgravity-simulation

Topics

Categories