Global Identification of Noncoding RNAs in S. cerevisiae
Description
Genome-wide detection of novel non-coding RNAs in S. cerevisiae by modulating an RNase P pathway through the depletion of a component RPP1. Nearly 400,000 36-mer oligonucleotide probes tiling the entire yeast genome including the mitochondrial chromosome with an average gap of 10 bases between two consecutive probes were synthesized on glass slides using a mask-less array synthesizer. RNA samples for hybridizing to the arrays were extracted from a conditional lethal allele of S. cerevisiae created by placing the RPP1 gene under control of GAL10 promoter. It allowed the expression of RPP1 in galactose-containing culture medium but suppressed its expression in glucose-containing medium. A wild-type isogenic strain was used as a control. Both strains were initially grown in galactose-containing medium and subsequently transferred and resuspended into glucose-containing medium. Eight arrays were hybridized with RNA extracted from the Rpp1-depleted cells at 0 4 7 12 16 21 30h and the control cell at 30h after initial transfer to glucose-containing medium.
Resources
| Name |
Format |
Description |
Link |
|
21 |
GeneLab Study Page |
https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-10 |
|
21 |
GeneLab Study Page |
https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-172 |
Tags
- normalization-data-transformation
- data-collection
- data-transformation
- p-gse4275-1
- bioassay_data_transformation
- p-gse4275-2
- nucleic-acid-hybridization
- rna-extraction
- labeling
- timepoint
- feature_extraction
- p-gse4275-3