Genomic and Proteomic characterization of Aspergillus niger isolated from the International Space Station
Description
Genomic and proteomic characterization of the Aspergillus niger isolate JSC-093350089 collected from U.S. segment surfaces of the International Space Station (ISS) is reported along with a comparison to the experimentally established strain ATCC 1015. Whole-genome sequencing of JSC-093350089 revealed enhanced genetic variance when compared to publicly available sequences of A. niger strains. Analysis of the isolate xe2 x80 x9a xc3 x84 xc3 xb4s proteome revealed significant differences in the molecular phenotype of JSC-093350089 including increased abundance of proteins involved in the A. niger starvation response oxidative stress resistance cell wall integrity and modulation and nutrient acquisition. Together these data reveal the existence of a distinct strain of A. niger onboard the ISS and provide insight into the molecular phenotype that is selected for by melanized fungal species inhabiting spacecraft environments.
Resources
| Name |
Format |
Description |
Link |
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21 |
GeneLab Study Page |
https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-260 |
Tags
- strain
- protein-extraction
- data-transformation
- extraction
- labeling
- growth-protocol
- spaceflight
- mass-spectrometry