During development, the Sku6 mutant roots engage different genes than wild type Col-0 roots, either on the ground or in spaceflight.

Description

The wild type Col-0 plants and the Sku-6 mutant plants of the Col-0 background were germinated on ISS or on the ground and the gene expression profiles in roots at 4 days or 8 days were established. The Sku6 gene (At2g03680) codes for a protein that localizes to microtubule within the cortical array and putatively acting as an intramolecular linker. The development of Col-0 and Sku-6 plants on orbit differs from that on the ground as demonstrated by the comparison of the gene expression profiles between 4 days old to 8 days old plant in the two environments. However the development of Sku-6 mutant plants also differs from Col-0 at either age and in either environment, suggesting the role of the genetic background in these developmental decisions. The 4 days old Sku-6 roots in orbit engaged more genes than the 4 days old Col-0 roots in orbit but also more than the 4 days old Sku-6 roots on the ground. Overall the 4 days old roots differentially expressed more genes in spaceflight relative to ground than the 8 days old roots of either genotype.

Resources

Name Format Description Link
5 https://www.ncbi.nlm.nih.gov%2Fgeo%2Fquery%2Facc.cgi%3Facc%3DGSE95582
5 https://lsda.jsc.nasa.gov%2FExperiment%2Fexper%2F13840
5 https://osdr.nasa.gov%2Fbio%2Frepo%2Fdata%2Fmissions%2FSpaceX-5
5 https://osdr.nasa.gov%2Fbio%2Frepo%2Fdata%2Fstudies%2FOSD-193
5 https://osdr.nasa.gov/bio/repo/data/missions/SpaceX-5
5 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE95582
5 https://lsda.jsc.nasa.gov/Experiment/exper/13840
5 https://osdr.nasa.gov/bio/repo/data/studies/OSD-193
5 https://osdr.nasa.gov/bio/repo/data/missions/SpaceX-5
5 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE95582
5 https://lsda.jsc.nasa.gov/Experiment/exper/13840
5 https://osdr.nasa.gov/bio/repo/data/studies/OSD-193
5 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE95582
5 https://lsda.jsc.nasa.gov/Experiment/exper/13840
5 https://osdr.nasa.gov/bio/repo/data/missions/SpaceX-5
5 https://osdr.nasa.gov/bio/repo/data/studies/OSD-193

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