Developing DNA Methods to Monitor Invasive Species and Biodiversity in Estuaries - NERRS/NSC(NERRS Science Collaborative)

Description

This project tested the use of eDNA methods to identify fish communities, assess biodiversity, and detect invasive crabs in different types of estuaries and connected streams. Samples were collected at Apalachicola, Great Bay, He’eai, Hudson, South Slough and Wells Reserves in 2018 and 2019. The project deployed two methods to analyze DNA in water samples: metabarcoding that identifies a wide range of species, and digital droplet Polymerase Chain Reaction (ddPCR) that identifies a single target organism. The team conducted baseline community assessments for fish at five reserve sites and conducted a survey of anadromous fish in a tributary stream at the sixth reserve. The project found that fish community and biodiversity assessments are well suited to eDNA applications, while invasive crabs are much harder to detect because they do not shed much DNA. The project developed protocols and recommendations for the collection, filtering, and extraction of eDNA samples at estuarine sites, and provided information which will support the design of sampling programs for fish communities.

Resources

Name Format Description Link
27 Processed datasets, data visualization tools, and related resources are available at the project webpage. https://estuarydna.org
33 NOAA Data Management Plan for this record on InPort. https://www.fisheries.noaa.gov/inportserve/waf/noaa/nos/ocm/dmp/pdf/54574.pdf
27 Processed datasets, data visualization tools, and related resources are available at the project webpage. https://estuarydna.org
0 This site provides a project overview and links to all associated products, including data. http://www.nerrssciencecollaborative.org/project/Watts17
0 View the complete metadata record on InPort for more information about this dataset. https://www.fisheries.noaa.gov/inport/item/54574
0 All genetic sequences generated by this project have been archived and made publicly available at the National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/search/all/?term=PRJNA667067
0 All genetic sequences generated by this project have been archived and made publicly available at the National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/search/all/?term=PRJNA667067
0 Global Change Master Directory (GCMD). 2024. GCMD Keywords, Version 19. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
0 This site provides a project overview and links to all associated products, including data. http://www.nerrssciencecollaborative.org/project/Watts17

Tags

  • 12s sequence data
  • nerrs
  • environmental dna
  • united states of america
  • office of coastal management
  • national ocean service
  • ecosystems
  • biosphere
  • south slough nerr
  • nh
  • invasive species
  • me
  • new hampshire
  • continent
  • noaa
  • great bay nerr
  • marine ecosystems
  • north america
  • doc/noaa/nos/ocm
  • maine
  • u.s. department of commerce
  • earth science
  • estuary
  • edna
  • monitoring
  • climate change
  • or
  • 18s sequence data
  • wells nerr
  • oregon

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