Description
Fathead minnows (Pimephales promelas) were exposed to 100 ug/L indomethacin, 200 ug/L ibuprofen, or 20 ug/L celecoxib for 96 h. Effects on cycloxygenase enzyme activity in ovary, prostaglandin F2alpha concentrations in plasma, 17beta-estradiol concentrations in plasma, and vitellogenin concentrations in plasma were measured. Gene expression in ovary samples was evaluated using a 15,000 probe oligonucleotide microarray. Transcriptomics data (raw data and normalized) are available through the National Center for Biotechnology Information, Gene Expression Omnibus (GEO), accession number GSE72976. Metabolite profiles in liver tissue were measured by proton nuclear magnetic resonance. In addition to these data, the data set also contains identification of differentially expressed genes, pathway enrichment and gene set enrichment analyes, ToxCast data for indomethacin and celecoxib, chemical-gene interaction data derived from the Comparative Toxicogenomics database, and results from Level 1, Level 2, and Level 3 SeqAPASS analyses that examine conservation of target proteins across species (https://seqapass.epa.gov/seqapass/).
This dataset is associated with the following publication:
Martinovic-Weigelt, D., A. Mehinto, G. Ankley , J. Berninger, T. Collette , J. Davis , N. Denslow, E. Durhan, E. Eid, D. Ekman , K. Jensen , M. Kahl , C. LaLone , Q. Teng , and D. Villeneuve. Derivation and evaluation of putative adverse outcome pathways for the effects of cyclooxygenase inhibitors on reproductive processes in female fish. TOXICOLOGICAL SCIENCES. Society of Toxicology, 156(2): 344-361, (2017).
Resources
| Name | Format | Description | Link |
|---|---|---|---|
| 10 ORD-013745 SeqAPASS level 3, cox1.xlsx | XLS | 10%20ORD-013745%20SeqAPASS%20level%203%2C%20cox1.xlsx | Open resource |
| 11 ORD-013745 SeqAPASS level 3, cox2.xlsx | XLS | 11%20ORD-013745%20SeqAPASS%20level%203%2C%20cox2.xlsx | Open resource |
| 1 ORD-013745 Differentially expressed genes.xlsx | XLS | 1%20ORD-013745%20Differentially%20expressed%20genes.xlsx | Open resource |
| 12 ORD-013745 MartinovicToxSCI_VTG_E2_COX_PGF.xlsx | XLS | 12%20ORD-013745%20MartinovicToxSCI_VTG_E2_COX_PGF.xlsx | Open resource |
| 13 ORD-013745 COX Inhib_Metabolomics_Diff Spec.xlsx | XLS | 13%20ORD-013745%20COX%20Inhib_Metabolomics_Diff%20Spec.xlsx | Open resource |
| 14 ORD-013745 COX Inhib_Metabolomics_PCA.xlsx | XLS | 14%20ORD-013745%20COX%20Inhib_Metabolomics_PCA.xlsx | Open resource |
| 2 ORD-013745 Pathway enrichment and GSEA.xlsx | XLS | 2%20ORD-013745%20Pathway%20enrichment%20and%20GSEA.xlsx | Open resource |
| 3 ORD-013745 Ingenuity Pathway Analyes.xlsx | XLS | 3%20ORD-013745%20Ingenuity%20Pathway%20Analyes.xlsx | Open resource |
| 4 ORD-013745 ToxCast data.xlsx | XLS | 4%20ORD-013745%20ToxCast%20data.xlsx | Open resource |
| 5 ORD-013745 CTD Chemical-gene interactions.xlsx | XLS | 5%20ORD-013745%20CTD%20Chemical-gene%20interactions.xlsx | Open resource |
| 6 ORD-013745 SeqAPASS level 1, cox1.csv | XLS | 6%20ORD-013745%20SeqAPASS%20level%201%2C%20cox1.csv | Open resource |
| 7 ORD-013745 SeqAPASS level 1, cox2.csv | XLS | 7%20ORD-013745%20SeqAPASS%20level%201%2C%20cox2.csv | Open resource |
| 8 ORD-013745 SeqAPASS level 2, cox1.csv | XLS | 8%20ORD-013745%20SeqAPASS%20level%202%2C%20cox1.csv | Open resource |
| 9 ORD-013745 SeqAPASS level 2, cox2.csv | XLS | 9%20ORD-013745%20SeqAPASS%20level%202%2C%20cox2.csv | Open resource |
| https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE72976 | Unknown | Open resource |