Description
This database gives hydrogen-deuterium exchange mass spectrometry (HDX-MS) data from measurements of three purified IgG1 glycoform samples, predominantly G0F, G2F, and SAF, in isolation and in complexation with the high-affinity receptor, Fc(gamma)RIa (CD64). The IgG1 antibody used in this study, aIL8hFc, is a murine-human chimeric IgG1, which inhibits IL-8 binding to human neutrophils. The working datasets comprise 17,750 deuterium uptake measurements in peptides from the receptor and the light and heavy chains of aIL8hFc obtained during 7 experiments, each comprising three runs of six exchange times. The data illuminate on the hydrogen exchange dynamics in isolated receptor, isolated IgG1 glycoforms, and receptor-IgG1 complexes.
Resources
| Name | Format | Description | Link |
|---|---|---|---|
| HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a. | CSV | HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a. | Open resource |
| SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a. | Text | Open resource | |
| HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc. | CSV | HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc. | Open resource |
| SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc. | Text | Open resource | |
| HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a. | CSV | HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a. | Open resource |
| SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a. | Text | Open resource | |
| HDX-MS data of peptides from isolated (apo-)Fc?R1a. | CSV | HDX-MS data of peptides from isolated (apo-)Fc?R1a. | Open resource |
| SHA256 File for HDX-MS data of peptides from isolated (apo-)Fc(gamma)R1a. | Text | Open resource | |
| HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform. | CSV | HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform. | Open resource |
| SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform. | Text | Open resource | |
| HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform. | CSV | HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform. | Open resource |
| SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform. | Text | Open resource | |
| HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform. | CSV | HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform. | Open resource |
| SHA256 File for HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform. | Text | Open resource | |
| HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc | CSV | HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc | Open resource |
| SHA256 File for HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc | Text | Open resource | |
| HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a . | CSV | HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a. | Open resource |
| SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a . | CSV | Open resource | |
| HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc | CSV | HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc | Open resource |
| SHA256 File HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc | Text | HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc | Open resource |
| HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a. | CSV | HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a. | Open resource |
| SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a. | Text | Open resource | |
| HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc | CSV | HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc | Open resource |
| SHA256 File for HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc | CSV | Open resource | |
| HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a. | CSV | HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a. | Open resource |
| SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a. | Text | SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a. | Open resource |
| HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc. | CSV | HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc. | Open resource |
| SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc. | Text | Open resource | |
| HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a. | CSV | HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a. | Open resource |
| SHA256 File for HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a. | Text | Open resource | |
| HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc. | CSV | HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc. | Open resource |
| SHA256 File for HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc. | CSV | Open resource | |
| Glossary of Protein Modifications | Glossary of post translational modifications present on peptides. | Open resource | |
| SHA256 File for Glossary of Protein Modifications | Text | Open resource | |
| Sequences of aILhFc and soluble FcgR1a (CD64a) | Sequences of aILhFc and soluble FcgR1a (CD64a) | Open resource | |
| SHA256 File for Sequences of aILhFc and soluble FcgR1a (CD64a) | Text | Open resource | |
| DOI Access for Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor | Unknown | Open resource |