Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor

Description

This database gives hydrogen-deuterium exchange mass spectrometry (HDX-MS) data from measurements of three purified IgG1 glycoform samples, predominantly G0F, G2F, and SAF, in isolation and in complexation with the high-affinity receptor, Fc(gamma)RIa (CD64). The IgG1 antibody used in this study, aIL8hFc, is a murine-human chimeric IgG1, which inhibits IL-8 binding to human neutrophils. The working datasets comprise 17,750 deuterium uptake measurements in peptides from the receptor and the light and heavy chains of aIL8hFc obtained during 7 experiments, each comprising three runs of six exchange times. The data illuminate on the hydrogen exchange dynamics in isolated receptor, isolated IgG1 glycoforms, and receptor-IgG1 complexes.

Resources

Name Format Description Link
47 https://data.nist.gov/od/ds/mds2-2365/Sequences%20of%20aILhFc%20and%20soluble%20FcgR1a%20%28CD64a%29.pdf.sha256
33 Sequences of aILhFc and soluble FcgR1a (CD64a) https://data.nist.gov/od/ds/mds2-2365/Sequences%20of%20aILhFc%20and%20soluble%20FcgR1a%20%28CD64a%29.pdf
8 https://data.nist.gov/od/ds/mds2-2365/Dataset%239_apo-G2F_heavy_chain.CSV.sha256
8 HDX-MS data of peptides from the heavy chain of isolated (apo-)G2F IgG1 aIL8hFc. https://data.nist.gov/od/ds/mds2-2365/Dataset%239_apo-G2F_heavy_chain.CSV
47 https://data.nist.gov/od/ds/mds2-2365/Dataset%2316_holo-FcgR1a%28SAF%29.CSV.sha256
8 HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G0F glycoform. https://data.nist.gov/od/ds/mds2-2365/Dataset%2314_holo-FcgR1a%28G0F%29.CSV
8 https://data.nist.gov/od/ds/mds2-2365/Dataset%232_holo-G0F_light_chain%28FcgR1a%29.CSV.sha256
47 https://data.nist.gov/od/ds/mds2-2365/Dataset%2310_holo-G2F_heavy_chain%28FcgR1a%29.CSV.sha256
8 HDX-MS data of peptides from the light chain of isolated (apo-)G0F IgG1 aIL8hFc https://data.nist.gov/od/ds/mds2-2365/Dataset%231_apo-G0F_light_chain.CSV
8 HDX-MS data of peptides from isolated (apo-)Fc?R1a. https://data.nist.gov/od/ds/mds2-2365/Dataset%2313_apo-FcgR1a.CSV
47 https://data.nist.gov/od/ds/mds2-2365/Dataset%2311_apo-SAF_heavy_chain.CSV.sha256
8 HDX-MS data of peptides from the light chain of isolated (apo-)SAF IgG1 aIL8hFc https://data.nist.gov/od/ds/mds2-2365/Dataset%235_apo-SAF_light_chain.CSV
47 https://data.nist.gov/od/ds/mds2-2365/Dataset%2314_holo-FcgR1a%28G0F%29.CSV.sha256
47 https://data.nist.gov/od/ds/mds2-2365/Dataset%234_holo-G2F_light_chain%28FcgR1a%29.CSV.sha256
8 HDX-MS data of peptides from the heavy chain of isolated (apo-)G0F IgG1 aIL8hFc. https://data.nist.gov/od/ds/mds2-2365/Dataset%237_apo-G0F_heavy_chain.CSV
8 https://data.nist.gov/od/ds/mds2-2365/Dataset%235_apo-SAF_light_chain.CSV.sha256
47 https://data.nist.gov/od/ds/mds2-2365/Dataset%231_apo-G0F_light_chain.CSV.sha256
8 HDX-MS data of peptides from the heavy chain of isolated (apo-)SAF IgG1 aIL8hFc. https://data.nist.gov/od/ds/mds2-2365/Dataset%2311_apo-SAF_heavy_chain.CSV
47 https://data.nist.gov/od/ds/mds2-2365/Dataset%2315_holo-FcgR1a%28G2F%29.CSV.sha256
8 HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc G2F glycoform. https://data.nist.gov/od/ds/mds2-2365/Dataset%2315_holo-FcgR1a%28G2F%29.CSV
47 https://data.nist.gov/od/ds/mds2-2365/Dataset%238_holo-G0F_heavy_chain%28FcgR1a%29.CSV.sha256
47 https://data.nist.gov/od/ds/mds2-2365/Dataset%2313_apo-FcgR1a.CSV.sha256
33 Glossary of post translational modifications present on peptides. https://data.nist.gov/od/ds/mds2-2365/Glossary%20of%20Protein%20Modifications.pdf
0 https://doi.org/10.18434/mds2-2365
8 HDX-MS data for peptides from (holo-)Fc(gamma)R1a in complexation with aIL8hFc SAF glycoform. https://data.nist.gov/od/ds/mds2-2365/Dataset%2316_holo-FcgR1a%28SAF%29.CSV
47 https://data.nist.gov/od/ds/mds2-2365/Dataset%237_apo-G0F_heavy_chain.CSV.sha256
8 HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc https://data.nist.gov/od/ds/mds2-2365/Dataset%233_apo-G2F_light_chain.CSV
47 HDX-MS data of peptides from the light chain of isolated (apo-)G2F IgG1 aIL8hFc https://data.nist.gov/od/ds/mds2-2365/Dataset%233_apo-G2F_light_chain.CSV.sha256
8 HDX-MS data for peptides from the light chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a. https://data.nist.gov/od/ds/mds2-2365/Dataset%234_holo-G2F_light_chain%28FcgR1a%29.CSV
8 HDX-MS data for peptides from the light chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a. https://data.nist.gov/od/ds/mds2-2365/Dataset%232_holo-G0F_light_chain%28FcgR1a%29.CSV
8 HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a. https://data.nist.gov/od/ds/mds2-2365/Dataset%236_holo-SAF_light_chain%28FcgR1a%29.CSV
8 HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G0F glycoform in complexation with Fc(gamma)R1a. https://data.nist.gov/od/ds/mds2-2365/Dataset%238_holo-G0F_heavy_chain%28FcgR1a%29.CSV
8 HDX-MS data for peptides from the heavy chain of holo-aIL8hFc G2F glycoform in complexation with Fc(gamma)R1a. https://data.nist.gov/od/ds/mds2-2365/Dataset%2310_holo-G2F_heavy_chain%28FcgR1a%29.CSV
47 https://data.nist.gov/od/ds/mds2-2365/Dataset%2312_holo-SAF_heavy_chain%28FcgR1a%29.CSV.sha256
8 HDX-MS data for peptides from the heavy chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a. https://data.nist.gov/od/ds/mds2-2365/Dataset%2312_holo-SAF_heavy_chain%28FcgR1a%29.CSV
47 SHA256 File for HDX-MS data for peptides from the light chain of holo-aIL8hFc SAF glycoform in complexation with Fc(gamma)R1a. https://data.nist.gov/od/ds/mds2-2365/Dataset%236_holo-SAF_light_chain%28FcgR1a%29.CSV.sha256
47 https://data.nist.gov/od/ds/mds2-2365/Glossary%20of%20Protein%20Modifications.pdf.sha256

Tags

  • hydrogen-deuterium-exchange
  • peptide
  • chromatography
  • hdx-ms
  • glycosylation
  • antibody-receptor-interaction
  • protein
  • proteomics
  • monoclonal-antibody
  • receptor
  • proteolysis
  • mass-spectrometry
  • precision

Topics

Categories