Data from: Host usage in <i>Aedes aegypti</i> collected from Houston, Texas and Phoenix, Arizona using whole mosquito third-generation sequencing blood meal analysis

Description

Aedes aegypti is the primary vector of several viruses of international public health concern, including Zika, dengue, yellow fever, and chikungunya. Their synanthropic ecology and establishment in tropical, sub-tropical, and temperate areas make Ae. aegypti one of the most medically relevant mosquito species in the world. While they have been reported to be highly anthropophilic, several studies indicate a broader host range. They are also reported to take multiple bloodmeals between gonotrophic cycles. This consumption of multiple bloodmeals makes determination of host usage difficult when using typical Sanger sequencing methods due to sequence overlap. In this study, we examined host usage of Ae. aegypti in Maricopa County, Arizona and Harris County, Texas, using a Nanopore-based third-generation sequencing protocol to alleviate this issue.

This repository contains files related to the methods described in this study, sequencing output metadata files, and the database files and the bioinformatic process used for the analysis of the sequencing data.

Resources

Name Format Description Link
alpha_sequencing_summary_FAW13400_12aa96af_1b0970c2.zip ZIP Open resource
bravo_sequencing_summary_FAW13414_1f13cb5e_a9c040c4.zip ZIP Open resource
gamma_sequencing_summary_FAT84617_fa79f416_253b38b8.zip ZIP Open resource
delta_sequencing_summary_FAL60047_3b7c9422_a93b80c2.zip ZIP Open resource
BOLD_All_Chordates.nin Unknown Open resource
BOLD_All_Chordates.nhr Unknown Open resource
BOLD_All_Chordates.nsq Unknown Open resource
Analysis_commands.txt Text Open resource
alpha_report_FAW13400_20230831_1730_12aa96af.html HTML Open resource
delta_report_FAL60047_20230607_1315_3b7c9422.html HTML Open resource
bravo_report_FAW13414_20230831_1729_1f13cb5e.html HTML Open resource
gamma_report_FAT84617_20231121_1622_fa79f416.html HTML Open resource
ReadMe.txt.txt Text Open resource
Sequencing_protocol.pdf PDF Open resource

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