CottonGen Primer3

Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:

• oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,

• PCR product size,

• positional constraints within the source (template) sequence, and

• possibilities for ectopic priming (amplifying the wrong sequence)

• many other constraints.

All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.

• Resource Title: Website Pointer for CottonGen Primer3.

  File Name: Web Page, url: https://www.cottongen.org/tools/primer3