Description
Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:
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oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,
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PCR product size,
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positional constraints within the source (template) sequence, and
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possibilities for ectopic priming (amplifying the wrong sequence)
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many other constraints.
All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.
Resources in this dataset:
Resource Title: Website Pointer for CottonGen Primer3.
File Name: Web Page, url: https://www.cottongen.org/tools/primer3
Resources
| Name | Format | Description | Link |
|---|---|---|---|
| https://www.cottongen.org/tools/primer3 | HTML | Open resource |