Construction of a Dual-enzyme, Subzero (-30 degree C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry

Description

This tutorial provides mechanical drawings, electrical schematics, parts lists, STL files for 3D-printed parts, IGS files, and instructions for automated machining necessary for construction of a dual-protease, subzero, liquid chromatography system for hydrogen-deuterium exchange mass spectrometry. Electrical schematics for construction of a multi-zone temperature controller that regulate to ±0.1 oC are also included in this tutorial.

Resources

Name Format Description Link
33 T-controller%20%28ver%202%29.pdf https://data.nist.gov/od/ds/mds2-2151/T-controller%20%28ver%202%29.pdf
47 https://data.nist.gov/od/ds/mds2-2151/T-controller%20%28ver%202%29.pdf.sha256
47 https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20T-controller.pdf.sha256
5 This file contains the STL files for the 3D-printed parts used to construct the Multi-Channel Valve Controller https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20Valve%20Controller%20STL%20files.7z
0 https://doi.org/10.18434/M32151
33 This file presents mechanical drawings, electrical schematics, parts lists, STL file inventory for 3D-printed parts, and instructions for construction of the Multi-Channel Valve Controller. https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20Valve%20Controller.pdf
47 https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20T-controller%20STL%20Files.7z.sha256
33 This file presents mechanical drawings, electrical schematics, parts lists, STL file inventory for 3D-printed parts, and instructions for construction of the Multi-channel Temperature Controller https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20T-controller.pdf
47 https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20Valve%20Controller%20STL%20files.7z.sha256
47 https://data.nist.gov/od/ds/mds2-2151/HDX-MS%20Analytical%20Instrument%20STL-IGS%20Files.7z.sha256
0 This is the compressed file containing the STL and IGS files that accompany the file, HDX-MS Analytical Instrument.pdf. https://data.nist.gov/od/ds/mds2-2151/HDX-MS%20Analytical%20Instrument%20STL-IGS%20Files.7z
33 This file presents mechanical drawings, electrical schematics, parts lists, lists of STL files for 3D-printed parts, lists of IGS files for automated machining, and instructions for construction of the HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 degree C https://data.nist.gov/od/ds/mds2-2151/HDX-MS%20Analytical%20Instrument.pdf
0 This addendum provides information for configuring the instrument with an 8-port valve in the Valve #2 location. The 8-port valve offers the ability to backflush the protease, trap, and analytical columns, which allows more rapid removal of protease aggregates from the top of each column. https://data.nist.gov/od/ds/mds2-2151/ADDENDUM%20for%20HDX.docx
47 https://data.nist.gov/od/ds/mds2-2151/ADDENDUM%20for%20HDX.docx.sha256
47 https://data.nist.gov/od/ds/mds2-2151/HDX-MS%20Analytical%20Instrument.pdf.sha256
47 https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20Valve%20Controller.pdf.sha256
0 STL Files for parts used to construct the Multi-channel Temperature Controller https://data.nist.gov/od/ds/mds2-2151/Multi-Channel%20T-controller%20STL%20Files.7z

Tags

  • temperature-control
  • hydrogen-deuterium-exchange
  • peptide
  • reference-material
  • liquid-chromatography
  • protein
  • proteomics
  • proteolysis
  • mass-spectrometry
  • precision

Topics

Categories