Description
This tutorial provides mechanical drawings, electrical schematics, parts lists, STL files for 3D-printed parts, IGS files, and instructions for automated machining necessary for construction of a dual-protease, subzero, liquid chromatography system for hydrogen-deuterium exchange mass spectrometry. Electrical schematics for construction of a multi-zone temperature controller that regulate to ±0.1 oC are also included in this tutorial.
Resources
| Name | Format | Description | Link |
|---|---|---|---|
| ADDENDUM for HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 oC | Unknown | This addendum provides information for configuring the instrument with an 8-port valve in the Valve #2 location. The 8-port valve offers the ability to backflush the protease, trap, and analytical columns, which allows more rapid removal of protease aggregates from the top of each column. | Open resource |
| SHA256 File for ADDENDUM for HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 oC | Text | Open resource | |
| STL and IGS files that accompany the file, HDX-MS Analytical Instrument.pdf | Unknown | This is the compressed file containing the STL and IGS files that accompany the file, HDX-MS Analytical Instrument.pdf. | Open resource |
| SHA256 File for STL and IGS files that accompany the file, HDX-MS Analytical Instrument.pdf | Text | Open resource | |
| HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 degree C | This file presents mechanical drawings, electrical schematics, parts lists, lists of STL files for 3D-printed parts, lists of IGS files for automated machining, and instructions for construction of the HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 degree C | Open resource | |
| SHA256 File for HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 degree C | Text | Open resource | |
| STL Files for parts used to construct the Multi-channel Temperature Controller | Unknown | STL Files for parts used to construct the Multi-channel Temperature Controller | Open resource |
| SHA256 File for STL Files for parts used to construct the Multi-channel Temperature Controller | Text | Open resource | |
| Construction information for the Multi-channel Temperature Controller | This file presents mechanical drawings, electrical schematics, parts lists, STL file inventory for 3D-printed parts, and instructions for construction of the Multi-channel Temperature Controller | Open resource | |
| SHA256 File for Construction information for the Multi-channel Temperature Controller | Text | Open resource | |
| Multi-Channel Valve Controller STL files | Binary | This file contains the STL files for the 3D-printed parts used to construct the Multi-Channel Valve Controller | Open resource |
| SHA256 File for Multi-Channel Valve Controller STL files | Text | Open resource | |
| Multi-Channel Valve Controller | This file presents mechanical drawings, electrical schematics, parts lists, STL file inventory for 3D-printed parts, and instructions for construction of the Multi-Channel Valve Controller. | Open resource | |
| SHA256 File for Multi-Channel Valve Controller | Text | Open resource | |
| PDF File | T-controller%20%28ver%202%29.pdf | Open resource | |
| Text File | Text | Open resource | |
| DOI Access for Construction of a Dual-enzyme, Subzero (-30 degree C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry | Unknown | Open resource |