Black sea bass, Centropristis striata, first 48 hour response to increased levels of carbon dioxide from 2013-07-13 to 2015-07-10 (NCEI Accession 0225335)

The effects of elevated levels of CO2 on black sea bass, especially during the early life stages when fish tend to be more sensitive to OA effects, was investigated. In this study, we exposed fertilized eggs to a range of CO2 levels (182.7 μatm- 2252.6 μatm) and measured % hatch, unhatch, and skeletal abnormalities after 48 hours of exposure. Adult male and female black sea bass were held in flowing seawater at ambient temperatures during the winters of 2012-2013, 2013-2014, and 2014-2015. Once fish came out of torpor, adults were fed squid during conditioning and spawning. Gamete development in fish occurred naturally and spawning took place in holding tanks in late July of all three years. Fertilized eggs were collected in screens placed at the seawater outflow and exposed to different levels of CO2.